m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05205
|
[1] | |||
Non-coding RNA
FTO-IT1
RBM15
lncRNA miRNA circRNA
Direct
Inhibition
m6A modification
SESN2
SESN2
RBM15
Methylation
 : m6A sites
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | RNA-binding motif protein 15 (RBM15) | WRITER | |||
| m6A Target | Sestrin-2 (SESN2) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | FTO intronic transcript 1 (FTO-IT1) | LncRNA | View Details | ||
| Regulated Target | RNA binding motif protein 15 (RBM15) | View Details | |||
| Crosstalk Relationship | ncRNA → m6A | Inhibition | |||
| Crosstalk Mechanism | ncRNAs directly impacts m6A modification through recruiting m6A regulator | ||||
| Crosstalk Summary | m6A RIP-seq analysis revealed that FTO-IT1 knockout increased mRNA m6A methylation of a subset of p53 transcriptional target genes (e.g., FAS, TP53INP1, and Sestrin-2 (SESN2)) and induced PCa cell cycle arrest and apoptosis. FTO-IT1 directly binds RBM15 and inhibits RBM15 binding, m6A methylation, and stability of p53 target mRNAs. | ||||
In-vitro Model |
HEK293T | Normal | Homo sapiens | CVCL_0063 | |
| 22Rv1 | Prostate carcinoma | Homo sapiens | CVCL_1045 | ||
| LNCaP C4-2 | Prostate carcinoma | Homo sapiens | CVCL_4782 | ||
| PC-3 | Prostate carcinoma | Homo sapiens | CVCL_0035 | ||
| LNCaP | Prostate carcinoma | Homo sapiens | CVCL_0395 | ||
| LAPC-4 | Prostate carcinoma | Homo sapiens | CVCL_4744 | ||
| In-vivo Model | 5 × 106 of mock KO or FTO-IT1 KO C4-2R cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice. For 22Rv1 xenografts, 5 × 106 of cells were mixed with Matrigel (50 μ l of PBS plus 50 μ l of Matrigel (BD Biosciences)) and injected subcutaneously into mice. | ||||