m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT03306				[1], [2]
Histone modification H3K18la Epigenetic Regulator METTL3 Direct Enhancement m⁶A modification XIST XIST METTL3 Methylation : m⁶A sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	X inactive specific transcript (XIST)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	Histone modification (HistMod)
Regulated Target	Histone H3 lysine 18 lactylation (H3K18la)				View Details
Downstream Gene	METTL3				View Details
Crosstalk Relationship	Histone modification → m6A			Enhancement
Crosstalk Mechanism	Histone modification directly impacts m6A modification through modulating the level of m6A regulator
Crosstalk Summary	LDHA knockdown-mediated inhibition of endogenous Histone H3 lysine 18 lactylation (H3K18la) suppressed osteogenic differentiation, a phenotype that was rescued by METTL3 overexpression. In conclusion, this study elucidates that histone lactylation-mediated upregulation of METTL3 promotes OLF progression through IGF2BP1-dependent m6A methylation of BMP2, providing novel insights into potential therapeutic strategies for OLF management. METTL3 regulates ossification of the posterior longitudinal ligament via the lncRNA X inactive specific transcript (XIST)/miR-302a-3p/USP8 axis.
Responsed Disease	Ossification of spinal ligaments		ICD-11: FA83		Disease Info
In-vitro Model	OLF-136	N.A.	Oryzias latipes		CVCL_3766

References

Ref 1	METTL3 regulated by histone lactylation promotes ossification of the ligamentum flavum by enhancing the m6A methylation of BMP2. Mol Med. 2025 Mar 25;31(1):118. doi: 10.1186/s10020-025-01173-x.
Ref 2	METTL3 Regulates Ossification of the Posterior Longitudinal Ligament via the lncRNA XIST/miR-302a-3p/USP8 Axis. Front Cell Dev Biol. 2021 Mar 5;9:629895. doi: 10.3389/fcell.2021.629895. eCollection 2021.