m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00564				[1], [2], [3]
m⁶A modification GAS5 GAS5 YTHDF3 : m⁶A sites Indirect Inhibition RNA modification CDKN1C CDKN1C NSUN2 Methylation : modification sites
m6A Regulator	YTH domain-containing family protein 3 (YTHDF3)			READER	Regulator Info
m6A Target	Growth arrest specific 5 (GAS5)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	RNA cytosine C(5)-methyltransferase NSUN2 (NSUN2)			WRITER	View Details
Regulated Target	Cyclin-dependent kinase inhibitor 1C (CDKN1C)				View Details
Crosstalk Relationship	m6A → m5C			Inhibition
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	YTHDF3 interacts with Growth arrest specific 5 (GAS5), increasing its m6A level and inhibiting its physical interaction with Cyclin-dependent kinase inhibitor 1C (CDKN1C), which was regulated by NSUN2-mediated m5C modification.
In-vitro Model	SGC-7901	Gastric carcinoma	Homo sapiens		CVCL_0520
	MGC-803	Gastric mucinous adenocarcinoma	Homo sapiens		CVCL_5334
	HCT 116	Colon carcinoma	Homo sapiens		CVCL_0291
	RKO	Colon carcinoma	Homo sapiens		CVCL_0504

References

Ref 1	RNA methyltransferase NSUN2 promotes gastric cancer cell proliferation by repressing p57(Kip2) by an m(5)C-dependent manner. Cell Death Dis. 2020 Apr 24;11(4):270. doi: 10.1038/s41419-020-2487-z.
Ref 2	Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m(6)A reader YTHDF3. Mol Cancer. 2019 Oct 16;18(1):143. doi: 10.1186/s12943-019-1079-y.
Ref 3	Long non-coding RNA GAS5 accelerates oxidative stress in melanoma cells by rescuing EZH2-mediated CDKN1C downregulation. Cancer Cell Int. 2020 Apr 9;20:116. doi: 10.1186/s12935-020-01167-1. eCollection 2020.