m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00522				[1], [2], [3]
RNA modification MIR221 MIR221 ADARB1 Methylation : modification sites Indirect Inhibition m⁶A modification FOXO3 FOXO3 ALKBH5 Demethylation : m⁶A sites
m6A Regulator	RNA demethylase ALKBH5 (ALKBH5)			ERASER	Regulator Info
m6A Target	Forkhead box protein O3 (FOXO3)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Double-stranded RNA-specific editase 1 (ADARB1)			WRITER	View Details
Regulated Target	MicroRNA 221 (MIR221)				View Details
Crosstalk Relationship	A-to-I → m6A			Inhibition
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	ADARB1 interacts with MicroRNA 221 (MIR221), increasing it's A-to-I level and inhibiting its physical interaction with Forkhead box protein O3 (FOXO3), which was regulated by ALKBH5-mediated m6A modification.
In-vitro Model	HCT 116	Colon carcinoma	Homo sapiens		CVCL_0291
	SW480	Colon adenocarcinoma	Homo sapiens		CVCL_0546
	U-118MG	Astrocytoma	Homo sapiens		CVCL_0633

References

Ref 1	m(6)A demethylase ALKBH5 inhibits cell proliferation and the metastasis of colorectal cancer by regulating the FOXO3/miR-21/SPRY2 axis. Am J Transl Res. 2021 Oct 15;13(10):11209-11222. eCollection 2021.
Ref 2	Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. Genome Biol. 2015 Jan 13;16(1):5. doi: 10.1186/s13059-014-0575-z.
Ref 3	circPAN3 exerts a profibrotic role via sponging miR-221 through FoxO3/ATG7-activated autophagy in a rat model of myocardial infarction. Life Sci. 2020 Sep 15;257:118015. doi: 10.1016/j.lfs.2020.118015. Epub 2020 Jul 3.