m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00513				[1], [2], [3]
RNA modification MIR18A MIR18A ADAR Methylation : modification sites Indirect Enhancement m⁶A modification PTEN PTEN ALKBH5 Demethylation : m⁶A sites
m6A Regulator	RNA demethylase ALKBH5 (ALKBH5)			ERASER	Regulator Info
m6A Target	Mutated in multiple advanced cancers 1 (PTEN)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Interferon-inducible protein 4 (ADAR1)			WRITER	View Details
Regulated Target	hsa-mir-18a				View Details
Crosstalk Relationship	A-to-I → m6A			Enhancement
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	ADAR1 interacts with hsa-mir-18a, increasing it's A-to-I level and promoting its physical interaction with Mutated in multiple advanced cancers 1 (PTEN), which was regulated by ALKBH5-mediated m6A modification.
In-vitro Model	BEAS-2B	Normal	Homo sapiens		CVCL_0168
	CAL-27	Tongue squamous cell carcinoma	Homo sapiens		CVCL_1107
	HN4	Clear cell renal cell carcinoma	Homo sapiens		CVCL_IS30

References

Ref 1	ALKBH5 promotes cadmium-induced transformation of human bronchial epithelial cells by regulating PTEN expression in an m6A-dependent manner. Ecotoxicol Environ Saf. 2021 Aug 23;224:112686. doi: 10.1016/j.ecoenv.2021.112686. Online ahead of print.
Ref 2	ADAR1 promotes the epithelial-to-mesenchymal transition and stem-like cell phenotype of oral cancer by facilitating oncogenic microRNA maturation. J Exp Clin Cancer Res. 2019 Jul 17;38(1):315. doi: 10.1186/s13046-019-1300-2.
Ref 3	Long Noncoding RNA WDFY3-AS2 Represses the Progression of Esophageal Cancer through miR-18a/PTEN Axis. J Oncol. 2021 Jun 5;2021:9951010. doi: 10.1155/2021/9951010. eCollection 2021.