m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00430				[1], [2], [3], [4], [5]
m⁶A modification MEG3 MEG3 METTL3 Methylation : m⁶A sites Indirect Inhibition RNA modification MIR21 MIR21 ADAR Methylation : modification sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	Maternally expressed 3 (MEG3)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Interferon-inducible protein 4 (ADAR1)			WRITER	View Details
Regulated Target	MicroRNA 21 (MIR21)				View Details
Crosstalk Relationship	m6A → A-to-I			Inhibition
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL3 interacts with Maternally expressed 3 (MEG3), increasing its m6A level and inhibiting its physical interaction with MicroRNA 21 (MIR21), which was regulated by ADAR1-mediated A-to-I modification.
In-vitro Model	Hep-G2	Hepatoblastoma	Homo sapiens		CVCL_0027
	Huh-7	Adult hepatocellular carcinoma	Homo sapiens		CVCL_0336
	HN4	Clear cell renal cell carcinoma	Homo sapiens		CVCL_IS30
	CAL-27	Tongue squamous cell carcinoma	Homo sapiens		CVCL_1107

References

Ref 1	m6A-Induced LncRNA MEG3 Suppresses the Proliferation, Migration and Invasion of Hepatocellular Carcinoma Cell Through miR-544b/BTG2 Signaling. Onco Targets Ther. 2021 Jun 15;14:3745-3755. doi: 10.2147/OTT.S289198. eCollection 2021.
Ref 2	The Role of ADAR1 and ADAR2 in the Regulation of miRNA-21 in Idiopathic Pulmonary Fibrosis. Lung. 2018 Aug;196(4):393-400. doi: 10.1007/s00408-018-0115-9. Epub 2018 Apr 10.
Ref 3	ADAR1 promotes the epithelial-to-mesenchymal transition and stem-like cell phenotype of oral cancer by facilitating oncogenic microRNA maturation. J Exp Clin Cancer Res. 2019 Jul 17;38(1):315. doi: 10.1186/s13046-019-1300-2.
Ref 4	Long non-coding RNA MEG3 promotes autophagy and apoptosis of nasopharyngeal carcinoma cells via PTEN up-regulation by binding to microRNA-21. J Cell Mol Med. 2021 Jan;25(1):61-72. doi: 10.1111/jcmm.15759. Epub 2020 Dec 17.
Ref 5	Long non?coding RNA MEG3 inhibits cell migration and invasion of non?small cell lung cancer cells by regulating the miR?21?5p/PTEN axis. Mol Med Rep. 2021 Mar;23(3):191. doi: 10.3892/mmr.2021.11830. Epub 2021 Jan 26.