m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00422				[1], [2], [3], [4], [5]
m⁶A modification XIST XIST METTL3 Methylation : m⁶A sites Indirect Enhancement RNA modification MIR21 MIR21 ADAR Methylation : modification sites
m6A Regulator	Methyltransferase-like 3 (METTL3)			WRITER	Regulator Info
m6A Target	X inactive specific transcript (XIST)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Interferon-inducible protein 4 (ADAR1)			WRITER	View Details
Regulated Target	MicroRNA 21 (MIR21)				View Details
Crosstalk Relationship	m6A → A-to-I			Enhancement
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL3 interacts with X inactive specific transcript (XIST), increasing its m6A level and promoting its physical interaction with MicroRNA 21 (MIR21), which was regulated by ADAR1-mediated A-to-I modification.
In-vitro Model	MGG8	Glioblastoma	Homo sapiens		CVCL_D1H4
	HEK293T	Normal	Homo sapiens		CVCL_0063
	HN4	Clear cell renal cell carcinoma	Homo sapiens		CVCL_IS30
	CAL-27	Tongue squamous cell carcinoma	Homo sapiens		CVCL_1107

References

Ref 1	N?-Methyladenosine Landscape of Glioma Stem-Like Cells: METTL3 Is Essential for the Expression of Actively Transcribed Genes and Sustenance of the Oncogenic Signaling. Genes (Basel). 2019 Feb 13;10(2):141. doi: 10.3390/genes10020141.
Ref 2	METTL3 Regulates Ossification of the Posterior Longitudinal Ligament via the lncRNA XIST/miR-302a-3p/USP8 Axis. Front Cell Dev Biol. 2021 Mar 5;9:629895. doi: 10.3389/fcell.2021.629895. eCollection 2021.
Ref 3	The Role of ADAR1 and ADAR2 in the Regulation of miRNA-21 in Idiopathic Pulmonary Fibrosis. Lung. 2018 Aug;196(4):393-400. doi: 10.1007/s00408-018-0115-9. Epub 2018 Apr 10.
Ref 4	ADAR1 promotes the epithelial-to-mesenchymal transition and stem-like cell phenotype of oral cancer by facilitating oncogenic microRNA maturation. J Exp Clin Cancer Res. 2019 Jul 17;38(1):315. doi: 10.1186/s13046-019-1300-2.
Ref 5	Long non-coding RNA X-inactive specific transcript silencing ameliorates primary graft dysfunction following lung transplantation through microRNA-21-dependent mechanism. EBioMedicine. 2020 Feb;52:102600. doi: 10.1016/j.ebiom.2019.102600. Epub 2020 Jan 22.