m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT00067
|
[1] | |||
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 : modification sites
Direct
Enhancement
m6A modification
SOX9
SOX9
METTL3
Methylation
 : m6A sites
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | Methyltransferase-like 3 (METTL3) | WRITER | |||
| m6A Target | Transcription factor SOX-9 (SOX9) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | RNA modification (RNAMod) >> 5-methylcytidine (m5C) | ||||
| Epigenetic Regulator | 5-methylcytosine rRNA methyltransferase NSUN4 (NSUN4) | WRITER | View Details | ||
| Regulated Target | Transcription factor SOX-9 (SOX9) | View Details | |||
| Crosstalk Relationship | m5C → m6A | Enhancement | |||
| Crosstalk Mechanism | RNA modification directly impacts m6A modification through recruiting m6A regulator and targeting the shared RNA | ||||
| Crosstalk Summary | BMSCs overexpressing METTL3 and NSUN4 can promote the repair of cartilage defects in vivo. m5C and m6A co-regulate the translation of Transcription factor SOX-9 (SOX9) in a YTHDF2-dependent manner during the chondrogenic differentiation of BMSCs, which provides a therapeutic target for clinical implications. | ||||
| Cell Process | Cell differentiation | ||||
| In-vivo Model | The rats were anaesthetized via strictly aseptic techniques during surgery. The knee joint was opened laterally by dislocating the patellar tendon, and a full thickness osteochondral defect (3-mm in diameter) was created in the trochlear groove of the femur. Then, approximately 107BMSCs were mixed with 1 ml hydrogel matrix (Coring, Cat#354250) and were injected into the defect. | ||||