m6A Target Gene Information
General Information of the m6A Target Gene (ID: M6ATAR00784)
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
LncRNA NORAD
can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Wilms tumor 1-associating protein (WTAP) [WRITER]
| Representative RNA-seq result indicating the expression of this target gene regulated by WTAP | ||
| Cell Line | Human umbilical vein endothelial cells | Homo sapiens |
|
Treatment: siWTAP HUVECs
Control: siControl HUVECs
|
GSE167067 | |
| Regulation |
  |
logFC: -7.45E-01 p-value: 4.90E-04 |
| More Results | Click to View More RNA-seq Results | |
| In total 1 item(s) under this regulator | ||||
| Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene | [1] | |||
| Response Summary | Subsequent loss- and gain-of-function experiments reveal WTAP is increased in senescent nucleus pulposus cells, and significantly promotes Long intergenic non-protein coding RNA 657 (NORAD) m6A modification. This study shows interruption of NORAD m6A modification or the NORAD/PUMILIO/E2F3 axis could serve as a potential therapeutic target to inhibit the senescence of NPCs and development of intervertebral disc degeneration(IVDD). | |||
| Responsed Disease | Intervertebral disc degeneration | ICD-11: FA80 | ||
| Pathway Response | Cellular senescence | hsa04218 | ||
| Cell Process | Cell senescence | |||
| In-vitro Model | Nucleus pulposus cells (NPCs) (Nucleus pulposus cells) | |||
| In-vivo Model | After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice. | |||
Intervertebral disc degeneration [ICD-11: FA80]
| In total 1 item(s) under this disease | ||||
| Experiment 1 Reporting the m6A-centered Disease Response | [1] | |||
| Response Summary | Subsequent loss- and gain-of-function experiments reveal WTAP is increased in senescent nucleus pulposus cells, and significantly promotes Long intergenic non-protein coding RNA 657 (NORAD) m6A modification. This study shows interruption of NORAD m6A modification or the NORAD/PUMILIO/E2F3 axis could serve as a potential therapeutic target to inhibit the senescence of NPCs and development of intervertebral disc degeneration(IVDD). | |||
| Responsed Disease | Intervertebral disc degeneration [ICD-11: FA80] | |||
| Target Regulator | Wilms tumor 1-associating protein (WTAP) | WRITER | ||
| Pathway Response | Cellular senescence | hsa04218 | ||
| Cell Process | Cell senescence | |||
| In-vitro Model | Nucleus pulposus cells (NPCs) (Nucleus pulposus cells) | |||
| In-vivo Model | After 50g male NORAD-KO or C57 mice at age of 8 weeks were anesthetized with 3% (w/v) pentobarbital (2 ml/kg) and grouped randomly, investigators blinded to the group allocation performed the experiment. The disc levels in rat tail (Co6/7, 7/8, and 8/9) were located by palpation on the coccygeal vertebrae and confirmed by trial radiography. Needles (33-G) were used to puncture the annulus fibrosus layer though the tail skin, in parallel to the end plates. To ensure that the needle did not penetrate too deeply , the length of the needle was pre-determined according to the dimensions of annulus fibrosus and the NP , which were measured in a preliminary experiment and found to be approximately 4 mm. Five kinds of solution were prepared for intradisc injection, including AA V vector, AAV containing shPUM1, AAV containing shPUM2 for Norad KO mice, AAV vector, AAV containing shE2F3, AAVcontaining OE-E2F3 for WT mice. | |||