m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00728)
Target Name Chloride intracellular channel protein 4 (CLIC4)
Synonyms
Intracellular chloride ion channel protein p64H1
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Gene Name CLIC4
Chromosomal Location 1p36.11
Family Chloride channel CLIC family
Function
Can insert into membranes and form poorly selective ion channels that may also transport chloride ions. Channel activity depends on the pH. Membrane insertion seems to be redox-regulated and may occur only under oxydizing conditions. Promotes cell-surface expression of HRH3. Has alternate cellular functions like a potential role in angiogenesis or in maintaining apical-basolateral membrane polarity during mitosis and cytokinesis. Could also promote endothelial cell proliferation and regulate endothelial morphogenesis (tubulogenesis).
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Gene ID 25932
Uniprot ID
CLIC4_HUMAN
HGNC ID
HGNC:13518
Ensembl Gene ID
ENSG00000169504
KEGG ID
hsa:25932
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
CLIC4 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Fat mass and obesity-associated protein (FTO) [ERASER]
 Regulator Info 
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary FTO suppresses PCa proliferation and metastasis through reducing the degradation of Chloride intracellular channel protein 4 (CLIC4) mRNA in an m6A dependent manner.
Target Regulation Up regulation
Responsed Disease Prostate cancer ICD-11: 2C82
 Disease Info 
In-vitro Model RWPE-1 Normal Homo sapiens CVCL_3791
PC-3 Prostate carcinoma Homo sapiens CVCL_0035
DU145 Prostate carcinoma Homo sapiens CVCL_0105
LNCaP C4-2 Prostate carcinoma Homo sapiens CVCL_4782
In-vivo Model Approximately 2×106 PCa cells (DU145 transfected with shFTO and shNC) were injected subcutaneously in mice. The tumor volume (V = (0.5*length*width2)) was measured with Vernier caliper every week. Ten mice were randomly divided into two groups, 2×106 cells transfected with shFTO and shNC were resuspended with 100 uL PBS and injected into the mouse tail vein to create a metastatic model. After 7 weeks, the mice were anesthetized, and D-luciferin (#D-Luciferin, Apexbio) was injected intraperitoneally, then used the IVIS imaging system (Caliper Life Sciences) to visualize the luciferase signal.
Prostate cancer [ICD-11: 2C82]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary FTO suppresses PCa proliferation and metastasis through reducing the degradation of Chloride intracellular channel protein 4 (CLIC4) mRNA in an m6A dependent manner.
Responsed Disease Prostate cancer [ICD-11: 2C82]
Target Regulator Fat mass and obesity-associated protein (FTO) ERASER
 Regulator Info 
Target Regulation Up regulation
In-vitro Model RWPE-1 Normal Homo sapiens CVCL_3791
PC-3 Prostate carcinoma Homo sapiens CVCL_0035
DU145 Prostate carcinoma Homo sapiens CVCL_0105
LNCaP C4-2 Prostate carcinoma Homo sapiens CVCL_4782
In-vivo Model Approximately 2×106 PCa cells (DU145 transfected with shFTO and shNC) were injected subcutaneously in mice. The tumor volume (V = (0.5*length*width2)) was measured with Vernier caliper every week. Ten mice were randomly divided into two groups, 2×106 cells transfected with shFTO and shNC were resuspended with 100 uL PBS and injected into the mouse tail vein to create a metastatic model. After 7 weeks, the mice were anesthetized, and D-luciferin (#D-Luciferin, Apexbio) was injected intraperitoneally, then used the IVIS imaging system (Caliper Life Sciences) to visualize the luciferase signal.
References
Ref 1 N6-methyladenosine demethylase FTO suppressed prostate cancer progression by maintaining CLIC4 mRNA stability. Cell Death Discov. 2022 Apr 9;8(1):184. doi: 10.1038/s41420-022-01003-7.