m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00631)
Target Name Homeobox-containing protein 1 (HMBOX1)
Synonyms
Homeobox telomere-binding protein 1; Telomere-associated homeobox-containing protein 1
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Gene Name HMBOX1
Chromosomal Location 8p21.1-p12
Function
Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA. Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation. Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres (By similarity). Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs). Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination. Might also be involved in the DNA damage response at telomeres.
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Gene ID 79618
Uniprot ID
HMBX1_HUMAN
HGNC ID
HGNC:26137
Ensembl Gene ID
ENSG00000147421
KEGG ID
hsa:79618
Full List of m6A Methylation Regulator of This Target Gene and Corresponding Disease/Drug Response(s)
HMBOX1 can be regulated by the following regulator(s), and cause disease/drug response(s). You can browse detail information of regulator(s) or disease/drug response(s).
Browse Regulator
Browse Disease
Wilms tumor 1-associating protein (WTAP) [WRITER]
 Regulator Info 
In total 1 item(s) under this regulator
Experiment 1 Reporting the m6A Methylation Regulator of This Target Gene [1]
Response Summary Homeobox-containing protein 1 (HMBOX1) was identified as the target gene of WTAP, WTAP/HMBOX1 regulated osteosarcoma growth and metastasis via PI3K/AKT pathway.
Target Regulation Down regulation
Responsed Disease Osteosarcoma ICD-11: 2B51
 Disease Info 
Pathway Response PI3K-Akt signaling pathway hsa04151
In-vitro Model U2OS Osteosarcoma Homo sapiens CVCL_0042
SJSA-1 Osteosarcoma Homo sapiens CVCL_1697
MG-63 Osteosarcoma Homo sapiens CVCL_0426
HOS Osteosarcoma Homo sapiens CVCL_0312
hFOB 1.19 Normal Homo sapiens CVCL_3708
143B Osteosarcoma Homo sapiens CVCL_2270
In-vivo Model Nude mice (4 weeks, male) were used for tumor model.For the subcutaneous tumor model, 1 × 106 shNC, shWTAP or shHMBOX1 or shWTAP/shHMBOX1 U2OS cells seeded into mice via subcutaneous injection. Tumor volume and tumor weight were measured to analyze tumor growth as previous described. For orthotopic xenograft tumor model, shNC, shWTAP, shHMBOX1, or shWTAP/shHMBOX1 U2OS cells were labeled with a luminescent dye and GFP, and injected into the cavity of the tibia of mice. Thirty days after injection, tumor growth was detected. For the metastasis model, the cells were injected into the tail vein, and the lung metastasis were detected 30 days after injection using in vivo bioluminescence imaging system.
Osteosarcoma [ICD-11: 2B51]
 Disease Info 
In total 1 item(s) under this disease
Experiment 1 Reporting the m6A-centered Disease Response [1]
Response Summary Homeobox-containing protein 1 (HMBOX1) was identified as the target gene of WTAP, WTAP/HMBOX1 regulated osteosarcoma growth and metastasis via PI3K/AKT pathway.
Responsed Disease Osteosarcoma [ICD-11: 2B51]
Target Regulator Wilms tumor 1-associating protein (WTAP) WRITER
 Regulator Info 
Target Regulation Down regulation
Pathway Response PI3K-Akt signaling pathway hsa04151
In-vitro Model U2OS Osteosarcoma Homo sapiens CVCL_0042
SJSA-1 Osteosarcoma Homo sapiens CVCL_1697
MG-63 Osteosarcoma Homo sapiens CVCL_0426
HOS Osteosarcoma Homo sapiens CVCL_0312
hFOB 1.19 Normal Homo sapiens CVCL_3708
143B Osteosarcoma Homo sapiens CVCL_2270
In-vivo Model Nude mice (4 weeks, male) were used for tumor model.For the subcutaneous tumor model, 1 × 106 shNC, shWTAP or shHMBOX1 or shWTAP/shHMBOX1 U2OS cells seeded into mice via subcutaneous injection. Tumor volume and tumor weight were measured to analyze tumor growth as previous described. For orthotopic xenograft tumor model, shNC, shWTAP, shHMBOX1, or shWTAP/shHMBOX1 U2OS cells were labeled with a luminescent dye and GFP, and injected into the cavity of the tibia of mice. Thirty days after injection, tumor growth was detected. For the metastasis model, the cells were injected into the tail vein, and the lung metastasis were detected 30 days after injection using in vivo bioluminescence imaging system.
References
Ref 1 WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m(6)A-dependent manner. Cell Death Dis. 2020 Aug 19;11(8):659. doi: 10.1038/s41419-020-02847-6.