m6A-centered Disease Response Information

General Information of the Disease (ID: M6ADIS0139)

Name	Corneal injury
ICD	ICD-11: NA06

Full List of Target Gene(s) of This m6A-centered Disease Response

DNA damage-inducible transcript 4 protein (DDIT4)

Target Info

In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene			[1]
Response Summary	METTL3 knockout in the limbal stem cells promotes the in vivo cell proliferation and migration, leading to the fast repair of corneal injury. In addition, m6A modification profiling identified stem cell regulatory factors AHNAK and DNA damage-inducible transcript 4 protein (DDIT4) as m6A targets.
Responsed Disease	Corneal injury [ICD-11: NA06.4]
Target Regulator	Methyltransferase-like 3 (METTL3)	WRITER	Regulator Info
Target Regulation	Up regulation
Cell Process	Cell proliferation
	Cell migration
In-vitro Model	CGC (Conjunctival goblet cells)
In-vivo Model	Mettl3^fl/wt mice were generated as previously described. Mettl3^fl/wt mice were crossed with K14CreER mice to obtain K14^creER/Mettl3^fl/fl (cKO) mice. Mettl3 cKO and control mice were injected with tamoxifen and then were subjected to corneal alkali burn treatment. The right eye was the experimental eye, and the left eye was the control eye. The mice were sacrificed at 24 hours, 7 days, 14 days, 35 days, and 56 days after injury. Six mice were taken from each period. Both eyes were removed, frozen in OCT (n = 4), fixed in 4% paraformaldehyde, and embedded in conventional paraffin (n = 2).

Neuroblast differentiation-associated protein AHNAK (AHNAK)

Target Info

In total 1 item(s) under this target gene
Experiment 1 Reporting the m6A-centered Disease Response by This Target Gene			[1]
Response Summary	METTL3 knockout in the limbal stem cells promotes the in vivo cell proliferation and migration, leading to the fast repair of corneal injury. In addition, m6A modification profiling identified stem cell regulatory factors Neuroblast differentiation-associated protein AHNAK (AHNAK) and DDIT4 as m6A targets.
Responsed Disease	Corneal injury [ICD-11: NA06.4]
Target Regulator	Methyltransferase-like 3 (METTL3)	WRITER	Regulator Info
Target Regulation	Up regulation
Cell Process	Cell proliferation
	Cell migration
In-vitro Model	CGC (Conjunctival goblet cells)
In-vivo Model	Mettl3^fl/wt mice were generated as previously described. Mettl3^fl/wt mice were crossed with K14CreER mice to obtain K14^creER/Mettl3^fl/fl (cKO) mice. Mettl3 cKO and control mice were injected with tamoxifen and then were subjected to corneal alkali burn treatment. The right eye was the experimental eye, and the left eye was the control eye. The mice were sacrificed at 24 hours, 7 days, 14 days, 35 days, and 56 days after injury. Six mice were taken from each period. Both eyes were removed, frozen in OCT (n = 4), fixed in 4% paraformaldehyde, and embedded in conventional paraffin (n = 2).

References

Ref 1	METTL3-Mediated m(6)A RNA Modification Regulates Corneal Injury Repair. Stem Cells Int. 2021 Oct 22;2021:5512153. doi: 10.1155/2021/5512153. eCollection 2021.