m6A Target Gene Information

General Information of the m6A Target Gene (ID: M6ATAR00997)

Target Name	Glutathione-specific gamma-glutamylcyclotransferase 1 (CHAC1)
Synonyms	Gamma-GCG 1; EC 4.3.2.7; Blocks Notch protein; Botch; Cation transport regulator-like protein 1 Click to Show/Hide
Gene Name	CHAC1
Chromosomal Location	15q15.1
Family	Gamma-glutamylcyclotransferase family, ChaC subfamily
Function	Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides (PubMed:27913623). Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade (PubMed:19109178). Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos (PubMed:22445366). Click to Show/Hide
Gene ID	79094
Uniprot ID	CHAC1_HUMAN
HGNC ID	HGNC:28680
KEGG ID	hsa:79094

Full List of Crosstalk(s) between m6A Modification and Epigenetic Regulation Related to This Regulator

Histone modification

m6A Regulator: RNA demethylase ALKBH5 (ALKBH5)

Regulator Info

In total 2 item(s) under this m6A regulator
Crosstalk ID: M6ACROT03472		Crosstalk Info
Epigenetic Regulator	Histone acetyltransferase p300 (P300)
Regulated Target	Histone H3 lysine 27 acetylation (H3K27ac)
Crosstalk relationship	Histone modification → m6A
Disease	Gastric cancer	Disease Info
Drug	cisplatin	Drug Info
Crosstalk ID: M6ACROT06023		Crosstalk Info
Epigenetic Regulator	CREB-binding protein (CREBBP)
Regulated Target	Histone H3 lysine 27 acetylation (H3K27ac)
Crosstalk relationship	Histone modification → m6A
Disease	Gastric cancer	Disease Info
Drug	cisplatin	Drug Info

RNA Modification Sequencing Data Associated with the Target (ID: M6ATAR00997)

Glutathione-specific gamma-glutamylcyclotransferase 1 (CHAC1)

5-methylcytidine (m5C)

In total 1 m6A sequence/site(s) in this target gene
mod ID: M5CSITE000395		Click to Show/Hide the Full List
mod site	chr15:40955919-40955920:+		[2]
Sequence	CTTTCTCAGTCCCTGCCTGTCTGCCAGCCTGCAGCTCTCCT
Seq Type List	Bisulfite-seq
Transcript ID List	rmsk_4316512; ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m5C_site_13818

N6-methyladenosine (m6A)

In total 29 m6A sequence/site(s) in this target gene
mod ID: M6ASITE022458		Click to Show/Hide the Full List
mod site	chr15:40953022-40953023:+		[3]
Sequence	TGCCCTGGACTCGACTTGGGACTGAGTCTGTGCCGCCTAGG
Motif Score	4.065041667
Cell/Tissue List	HeLa; A549; HepG2; GM12878; HEK293A-TOA; iSLK
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000444189.7
External Link	RMBase: m6A_site_272084
mod ID: M6ASITE022459		Click to Show/Hide the Full List
mod site	chr15:40953052-40953053:+		[3]
Sequence	TGCCGCCTAGGCTGGCAGGGACTCGGCCAGATGCACTAGCG
Motif Score	4.065041667
Cell/Tissue List	HeLa; A549; HepG2; GM12878; HEK293T; HEK293A-TOA; iSLK
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000444189.7
External Link	RMBase: m6A_site_272085
mod ID: M6ASITE022460		Click to Show/Hide the Full List
mod site	chr15:40953099-40953100:+		[3]
Sequence	CTGGGCTGACACCGCCTGGGACTCGCTCGCCGTACCTCCAA
Motif Score	4.065041667
Cell/Tissue List	HeLa; A549; HEK293T; HEK293A-TOA; iSLK
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000444189.7
External Link	RMBase: m6A_site_272086
mod ID: M6ASITE022461		Click to Show/Hide the Full List
mod site	chr15:40953539-40953540:+		[3]
Sequence	TTTCCGTGCCCACGCCGGAGACCAGCCCCGGAGGCCGCCTG
Motif Score	2.876744048
Cell/Tissue List	HeLa; A549; MT4; Jurkat; CD4T; GSC-11; HEK293T; HEK293A-TOA; iSLK; MSC; TIME; HEC-1-A; MM6
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000617768.5; ENST00000444189.7
External Link	RMBase: m6A_site_272087
mod ID: M6ASITE022462		Click to Show/Hide the Full List
mod site	chr15:40953608-40953609:+		[3]
Sequence	GCAGGAGTCTGCAGCCCCGAACACCCCGCCCACCTCGCAGT
Motif Score	2.951386905
Cell/Tissue List	HeLa; A549; hESC-HEK293T; MT4; Jurkat; CD4T; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; endometrial; HEC-1-A; NB4; MM6
Seq Type List	m6A-seq; MeRIP-seq; MAZTER-seq
Transcript ID List	ENST00000617768.5; ENST00000444189.7
External Link	RMBase: m6A_site_272088
mod ID: M6ASITE022463		Click to Show/Hide the Full List
mod site	chr15:40953785-40953786:+		[4]
Sequence	CCGCCGTTTCTGGCAGGGAGACACCTTCCATCGGGGCAGCG
Motif Score	2.897386905
Cell/Tissue List	HeLa; HEK293T; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000444189.7; ENST00000617768.5
External Link	RMBase: m6A_site_272089
mod ID: M6ASITE022464		Click to Show/Hide the Full List
mod site	chr15:40955550-40955551:+		[3]
Sequence	CTATGTGGCCACCCCACAGAACCCTGGTTACCTGGGCCCTG
Motif Score	2.930744048
Cell/Tissue List	HeLa; HEK293T; HepG2; U2OS; Huh7; TIME; iSLK; MSC; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000444189.7; ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272090
mod ID: M6ASITE022465		Click to Show/Hide the Full List
mod site	chr15:40955625-40955626:+		[5]
Sequence	CTGCCGGGGCTTCTCCGGCCACAACCTTGAATACTTGCTGC
Motif Score	2.053113095
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000487220.1; ENST00000617768.5; ENST00000444189.7
External Link	RMBase: m6A_site_272091
mod ID: M6ASITE022466		Click to Show/Hide the Full List
mod site	chr15:40955655-40955656:+		[3]
Sequence	ATACTTGCTGCGTCTGGCAGACTTCATGCAGCTCTGTGGGC
Motif Score	3.319380952
Cell/Tissue List	HeLa; HEK293T; A549; HepG2; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; CD8T; MM6; Huh7; Jurkat; CD4T; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A; NB4
Seq Type List	m6A-seq; MeRIP-seq; m6A-CLIP/IP
Transcript ID List	ENST00000617768.5; ENST00000487220.1; ENST00000444189.7
External Link	RMBase: m6A_site_272092
mod ID: M6ASITE022467		Click to Show/Hide the Full List
mod site	chr15:40955688-40955689:+		[6]
Sequence	CTGTGGGCCTCAGGCGCAGGACGAGCACCTGGCAGCCATCG
Motif Score	3.616982143
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000487220.1; ENST00000444189.7; ENST00000617768.5
External Link	RMBase: m6A_site_272093
mod ID: M6ASITE022468		Click to Show/Hide the Full List
mod site	chr15:40955807-40955808:+		[3]
Sequence	TGCGGGGAGTGCTCATGTGGACATCAGGGCCAGACACCCAC
Motif Score	3.643047619
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; hESC-HEK293T; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; LCLs; H1299; MM6; Huh7; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A; GSCs; NB4
Seq Type List	m6A-seq; MeRIP-seq; MAZTER-seq
Transcript ID List	ENST00000444189.7; ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272094
mod ID: M6ASITE022469		Click to Show/Hide the Full List
mod site	chr15:40955820-40955821:+		[3]
Sequence	CATGTGGACATCAGGGCCAGACACCCACTCCAGTGCACAAG
Motif Score	2.897386905
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; LCLs; H1299; MM6; Huh7; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A; GSCs; NB4
Seq Type List	m6A-seq; MeRIP-seq; DART-seq
Transcript ID List	ENST00000487220.1; ENST00000444189.7; ENST00000617768.5
External Link	RMBase: m6A_site_272095
mod ID: M6ASITE022470		Click to Show/Hide the Full List
mod site	chr15:40955836-40955837:+		[6]
Sequence	CCAGACACCCACTCCAGTGCACAAGACAGACTTGCGACCGC
Motif Score	2.830589286
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272096
mod ID: M6ASITE022471		Click to Show/Hide the Full List
mod site	chr15:40955841-40955842:+		[3]
Sequence	CACCCACTCCAGTGCACAAGACAGACTTGCGACCGCTTGAG
Motif Score	2.897386905
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; hESC-HEK293T; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; LCLs; H1299; MM6; Huh7; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A; GSCs; NB4
Seq Type List	m6A-seq; MeRIP-seq; DART-seq; MAZTER-seq
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272097
mod ID: M6ASITE022472		Click to Show/Hide the Full List
mod site	chr15:40955845-40955846:+		[7]
Sequence	CACTCCAGTGCACAAGACAGACTTGCGACCGCTTGAGCCCA
Motif Score	3.319380952
Cell/Tissue List	CD8T
Seq Type List	m6A-CLIP/IP
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272098
mod ID: M6ASITE022473		Click to Show/Hide the Full List
mod site	chr15:40955945-40955946:+		[5]
Sequence	GCCTGCAGCTCTCCTGCTTGACACTGACTTACTACTTGAAA
Motif Score	2.859755952
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272099
mod ID: M6ASITE022474		Click to Show/Hide the Full List
mod site	chr15:40955947-40955948:+		[6]
Sequence	CTGCAGCTCTCCTGCTTGACACTGACTTACTACTTGAAACT
Motif Score	2.506922619
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272100
mod ID: M6ASITE022475		Click to Show/Hide the Full List
mod site	chr15:40955951-40955952:+		[6]
Sequence	AGCTCTCCTGCTTGACACTGACTTACTACTTGAAACTTTAT
Motif Score	3.28175
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272101
mod ID: M6ASITE022476		Click to Show/Hide the Full List
mod site	chr15:40955955-40955956:+		[6]
Sequence	CTCCTGCTTGACACTGACTTACTACTTGAAACTTTATTTAT
Motif Score	2.494845238
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272102
mod ID: M6ASITE022477		Click to Show/Hide the Full List
mod site	chr15:40955958-40955959:+		[6]
Sequence	CTGCTTGACACTGACTTACTACTTGAAACTTTATTTATTGC
Motif Score	2.500660714
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272103
mod ID: M6ASITE022478		Click to Show/Hide the Full List
mod site	chr15:40955965-40955966:+		[3]
Sequence	ACACTGACTTACTACTTGAAACTTTATTTATTGCACCATGT
Motif Score	2.627720238
Cell/Tissue List	HeLa; HEK293T; A549; HepG2; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; CD8T; H1299; MM6; Huh7; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq; m6A-CLIP/IP
Transcript ID List	ENST00000487220.1; ENST00000617768.5
External Link	RMBase: m6A_site_272104
mod ID: M6ASITE022479		Click to Show/Hide the Full List
mod site	chr15:40956019-40956020:+		[3]
Sequence	AGGTGGAGGGCCTGCCCTGGACACAGGGGCCCTGCTGAGCA
Motif Score	3.643047619
Cell/Tissue List	HeLa; HEK293T; A549; hESC-HEK293T; HepG2; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; CD8T; H1299; Huh7; Jurkat; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq; MAZTER-seq; m6A-CLIP/IP
Transcript ID List	ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272105
mod ID: M6ASITE022480		Click to Show/Hide the Full List
mod site	chr15:40956056-40956057:+		[3]
Sequence	AGCAGTGGCCCCATCCTGGAACTTGACCAGATTCCCCCCAG
Motif Score	3.373380952
Cell/Tissue List	HeLa; HEK293T; A549; HepG2; U2OS; H1A; H1B; hESCs; fibroblasts; GM12878; CD8T; MT4; H1299; Huh7; Jurkat; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq; m6A-CLIP/IP
Transcript ID List	ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272106
mod ID: M6ASITE022481		Click to Show/Hide the Full List
mod site	chr15:40956061-40956062:+		[6]
Sequence	TGGCCCCATCCTGGAACTTGACCAGATTCCCCCCAGTGCTG
Motif Score	2.839113095
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000617768.5; ENST00000487220.1
External Link	RMBase: m6A_site_272107
mod ID: M6ASITE022482		Click to Show/Hide the Full List
mod site	chr15:40956251-40956252:+		[3]
Sequence	GGAGAAGGAGCCCTGTAAGGACTGAGGCCCCGGCCAGCCCT
Motif Score	4.065041667
Cell/Tissue List	HeLa; HEK293T; A549; HepG2; H1A; H1B; hESCs; fibroblasts; CD8T; MM6; Huh7; Jurkat; CD4T; peripheral-blood; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq; m6A-CLIP/IP
Transcript ID List	ENST00000617768.5
External Link	RMBase: m6A_site_272108
mod ID: M6ASITE022483		Click to Show/Hide the Full List
mod site	chr15:40956317-40956318:+		[3]
Sequence	GAGCTGGAGCTGATGCCTGGACACAGCTGCTGAGCCTGGCC
Motif Score	3.643047619
Cell/Tissue List	HeLa; HEK293T; A549; hESC-HEK293T; HepG2; H1A; H1B; MM6; Huh7; Jurkat; CD4T; peripheral-blood; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq; MAZTER-seq
Transcript ID List	ENST00000617768.5
External Link	RMBase: m6A_site_272109
mod ID: M6ASITE022484		Click to Show/Hide the Full List
mod site	chr15:40956392-40956393:+		[6]
Sequence	CTCTGTCTGTCTGTCTATCTACTTGTCTGTCTGGGCCACTC
Motif Score	2.500660714
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	rmsk_4316513; ENST00000617768.5
External Link	RMBase: m6A_site_272110
mod ID: M6ASITE022485		Click to Show/Hide the Full List
mod site	chr15:40956485-40956486:+		[6]
Sequence	ACCTTCCCAGTCTGTCCCATACTGTTACCCATAAAACTATC
Motif Score	2.53247619
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000617768.5
External Link	RMBase: m6A_site_272111
mod ID: M6ASITE022486		Click to Show/Hide the Full List
mod site	chr15:40956500-40956501:+		[3]
Sequence	CCCATACTGTTACCCATAAAACTATCTCTTTATCTGTGCTG
Motif Score	2.627720238
Cell/Tissue List	HeLa; Huh7
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000617768.5
External Link	RMBase: m6A_site_272112

Ref 1	Analysis of human cytochrome P450 2C8 substrate specificity using a substrate pharmacophore and site-directed mutants. Biochemistry. 2004 Dec 14;43(49):15379-92. doi: 10.1021/bi0489309.
Ref 2	5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader. Cell Res. 2017 May;27(5):606-625. doi: 10.1038/cr.2017.55. Epub 2017 Apr 18.
Ref 3	VIRMA mediates preferential m(6)A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell Discov. 2018 Feb 27;4:10. doi: 10.1038/s41421-018-0019-0. eCollection 2018.
Ref 4	RNA m(6)A methylation regulates the epithelial mesenchymal transition of cancer cells and translation of Snail. Nat Commun. 2019 May 6;10(1):2065. doi: 10.1038/s41467-019-09865-9.
Ref 5	Deciphering the "m(6)A Code" via Antibody-Independent Quantitative Profiling. Cell. 2019 Jul 25;178(3):731-747.e16. doi: 10.1016/j.cell.2019.06.013. Epub 2019 Jun 27.
Ref 6	DART-seq: an antibody-free method for global m(6)A detection. Nat Methods. 2019 Dec;16(12):1275-1280. doi: 10.1038/s41592-019-0570-0. Epub 2019 Sep 23.
Ref 7	A majority of m6A residues are in the last exons, allowing the potential for 3' UTR regulation. Genes Dev. 2015 Oct 1;29(19):2037-53. doi: 10.1101/gad.269415.115. Epub 2015 Sep 24.

m6A Target Gene Information

Cite M6AREG

Correspondence

Prof. Feng ZHU

Prof. Shuiping Liu

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