Epigenetic Target Information

General Information of the Epigenetic Target (ID: EPITAR00502)

Target Name	Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (ALKBH7)
Synonyms	Alkylated DNA repair protein alkB homolog 7; Spermatogenesis cell proliferation-related protein; Spermatogenesis-associated protein 11; ABH7; SPATA11 Click to Show/Hide
Gene Name	ALKBH7
Sequence	MAGTGLLALRTLPGPSWVRGSGPSVLSRLQDAAVVRPGFLSTAEEETLSRELEPELRRRRYEYDHWDAAIHGFRETEKSRWSEASRAILQRVQAAAFGPGQTLLSSVHVLDLEARGYIKPHVDSIKFCGATIAGLSLLSPSVMRLVHTQEPGEWLELLLEPGSLYILRGSARYDFSHEILRDEESFFGERRIPRGRRISVICRSLPEGMGPGESGQPPPAC Click to Show/Hide
Family	AlkB family.
Function	May function as protein hydroxylase; can catalyze auto-hydroxylation at Leu-110 (in vitro), but this activity may be due to the absence of the true substrate (PubMed:25122757). Required to induce programmed necrosis in response to DNA damage caused by cytotoxic alkylating agents. Acts by triggering the collapse of mitochondrial membrane potential and loss of mitochondrial function that leads to energy depletion and cell death (PubMed:23666923). ALKBH7-mediated necrosis is probably required to prevent the accumulation of cells with DNA damage (PubMed:23666923). Does not display DNA demethylase activity (PubMed:23666923). Involved in fatty acid metabolism (By similarity). {ECO:0000250\|UniProtKB:Q9D6Z0, ECO:0000269\|PubMed:23666923, ECO:0000269\|PubMed:25122757}. Click to Show/Hide
Gene ID	84266
HGNC ID	HGNC:21306
Ensembl Gene ID	ENSG00000125652
KEGG ID	hsa:84266
Chromosomal Location	19p13.3

RNA Modification Sequencing Data Associated with the Target (ID: EPITAR00502)

Alpha-ketoglutarate-dependent dioxygenase alkB homolog 7, mitochondrial (ALKBH7)

N4-acetylcytidine (ac4C)

In total 1 m6A sequence/site(s) in this target gene
mod ID: AC4SITE000246		Click to Show/Hide the Full List
mod site	chr19:6372891-6372892:+
Sequence	GTGCGAGGCTCGGGCCCTTCCGTGCTGAGCCGCCTGCAGGA
Cell/Tissue List	DLD1
Seq Type List	acRIP-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: ac4C_site_934

5-methylcytidine (m5C)

In total 2 m6A sequence/site(s) in this target gene
mod ID: M5CSITE001655		Click to Show/Hide the Full List
mod site	chr19:6372830-6372831:+
Sequence	CTCCGGGATTATGGCCGGGACTGGGCTGCTGGCGCTGCGGA
Cell/Tissue List	testis
Seq Type List	Bisulfite-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: m5C_site_22250
mod ID: M5CSITE001656		Click to Show/Hide the Full List
mod site	chr19:6374494-6374495:+		[1]
Sequence	CCACCATCGCCGGCCTGTCTCTCCTGTCTCCCAGCGTTATG
Seq Type List	Bisulfite-seq
Transcript ID List	ENST00000245812.8; ENST00000599849.1; ENST00000596657.1
External Link	RMBase: m5C_site_22251

N6-methyladenosine (m6A)

In total 19 m6A sequence/site(s) in this target gene
mod ID: M6ASITE038771		Click to Show/Hide the Full List
mod site	chr19:6372829-6372830:+		[2]
Sequence	GCTCCGGGATTATGGCCGGGACTGGGCTGCTGGCGCTGCGG
Motif Score	4.065041667
Cell/Tissue List	HeLa; MM6; CD4T; GSC-11; HEK293T
Seq Type List	m6A-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: m6A_site_414158
mod ID: M6ASITE038772		Click to Show/Hide the Full List
mod site	chr19:6372972-6372973:+		[2]
Sequence	GAGGAGACGCTGAGCCGAGAACTGGAGCCCGAGCTGCGCCG
Motif Score	3.373380952
Cell/Tissue List	HeLa; HepG2; MT4; A549; MM6; CD4T; GSC-11; HEK293T; endometrial; HEC-1-A; NB4
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: m6A_site_414159
mod ID: M6ASITE038773		Click to Show/Hide the Full List
mod site	chr19:6373706-6373707:+		[2]
Sequence	TGGGGGCGGAGATAGGGGAGACACATGCTGGGATGGGGCAG
Motif Score	2.897386905
Cell/Tissue List	HeLa; A549; MM6; HEK293T; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000599849.1; ENST00000596657.1; ENST00000245812.8
External Link	RMBase: m6A_site_414160
mod ID: M6ASITE038774		Click to Show/Hide the Full List
mod site	chr19:6374223-6374224:+		[2]
Sequence	CCATCCACGGCTTCCGAGAGACAGAGAAGTCGCGCTGGTCA
Motif Score	2.897386905
Cell/Tissue List	HeLa; HepG2; A549; MM6; GSC-11; HEK293T; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000245812.8; ENST00000599849.1; ENST00000596657.1
External Link	RMBase: m6A_site_414161
mod ID: M6ASITE038775		Click to Show/Hide the Full List
mod site	chr19:6374301-6374302:+		[2]
Sequence	CCGCCTTTGGCCCCGGCCAGACCCTGCTCTCCTCCGTGCAC
Motif Score	2.876744048
Cell/Tissue List	HeLa; HepG2; MM6; peripheral-blood; HEK293T; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000245812.8; ENST00000599849.1; ENST00000596657.1
External Link	RMBase: m6A_site_414162
mod ID: M6ASITE038776		Click to Show/Hide the Full List
mod site	chr19:6374329-6374330:+		[2]
Sequence	CTCCTCCGTGCACGTGCTGGACCTGGAAGCCCGCGGCTACA
Motif Score	3.622404762
Cell/Tissue List	HeLa; HepG2; MM6; peripheral-blood; HEK293T; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000599849.1; ENST00000245812.8; ENST00000596657.1
External Link	RMBase: m6A_site_414163
mod ID: M6ASITE038777		Click to Show/Hide the Full List
mod site	chr19:6374347-6374348:+		[3]
Sequence	GGACCTGGAAGCCCGCGGCTACATCAAGCCCCACGTGGACA
Motif Score	2.078666667
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000596657.1; ENST00000599849.1; ENST00000245812.8
External Link	RMBase: m6A_site_414164
mod ID: M6ASITE038778		Click to Show/Hide the Full List
mod site	chr19:6374365-6374366:+		[2]
Sequence	CTACATCAAGCCCCACGTGGACAGCATCAAGGTGGGCAGAG
Motif Score	3.643047619
Cell/Tissue List	HeLa; HepG2; MM6; peripheral-blood; HEK293T; endometrial; HEC-1-A
Seq Type List	m6A-seq
Transcript ID List	ENST00000245812.8; ENST00000599849.1; ENST00000596657.1
External Link	RMBase: m6A_site_414165
mod ID: M6ASITE038779		Click to Show/Hide the Full List
mod site	chr19:6374525-6374526:+		[4]
Sequence	CAGCGTTATGCGGCTGGTGCACACCCAGGAGCCGGGGGAGT
Motif Score	2.830589286
Cell/Tissue List	kidney; hESC-HEK293T
Seq Type List	m6A-REF-seq; MAZTER-seq
Transcript ID List	ENST00000245812.8; ENST00000599849.1; ENST00000596657.1
External Link	RMBase: m6A_site_414166
mod ID: M6ASITE038780		Click to Show/Hide the Full List
mod site	chr19:6374553-6374554:+		[2]
Sequence	GAGCCGGGGGAGTGGCTGGAACTCTTGCTGGAGCCGGGCTC
Motif Score	3.373380952
Cell/Tissue List	HeLa; HEK293T
Seq Type List	m6A-seq
Transcript ID List	ENST00000596657.1; ENST00000245812.8; ENST00000599849.1
External Link	RMBase: m6A_site_414167
mod ID: M6ASITE038781		Click to Show/Hide the Full List
mod site	chr19:6374579-6374580:+		[4]
Sequence	GCTGGAGCCGGGCTCCCTCTACATCCTTAGGTACCTCCATC
Motif Score	2.078666667
Cell/Tissue List	kidney; hESC-HEK293T
Seq Type List	m6A-REF-seq; MAZTER-seq
Transcript ID List	ENST00000245812.8; ENST00000596657.1; ENST00000599849.1
External Link	RMBase: m6A_site_414168
mod ID: M6ASITE038782		Click to Show/Hide the Full List
mod site	chr19:6374827-6374828:+		[5]
Sequence	CAGGGGCTCAGCCCGTTATGACTTCTCCCATGAGATCCTTC
Motif Score	3.28175
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000245812.8; ENST00000596657.1; ENST00000599849.1
External Link	RMBase: m6A_site_414169
mod ID: M6ASITE038783		Click to Show/Hide the Full List
mod site	chr19:6374951-6374952:+		[2]
Sequence	ATGGGGCCAGGGGAGTCTGGACAGCCGCCCCCAGCCTGCTG
Motif Score	3.643047619
Cell/Tissue List	HeLa; HepG2; A549; HEK293T; MM6; peripheral-blood; HEK293A-TOA; endometrial
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000599849.1; ENST00000245812.8; ENST00000596657.1
External Link	RMBase: m6A_site_414170
mod ID: M6ASITE038784		Click to Show/Hide the Full List
mod site	chr19:6374990-6374991:+		[2]
Sequence	TGACCCCCAGCTTTCTACAGACACCAGATTTGTGAATAAAG
Motif Score	2.897386905
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; H1A; H1B; hESCs; MM6; Huh7; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; TIME; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000245812.8; ENST00000596657.1; ENST00000599849.1
External Link	RMBase: m6A_site_414171
mod ID: M6ASITE038785		Click to Show/Hide the Full List
mod site	chr19:6375022-6375023:+		[2]
Sequence	TGAATAAAGTTGGGGAATGGACAGCCTAACTGGGACATTGC
Motif Score	3.643047619
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; hESC-HEK293T; H1A; H1B; hESCs; MM6; Huh7; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; TIME; endometrial; HEC-1-A; AML
Seq Type List	m6A-seq; MeRIP-seq; MAZTER-seq; miCLIP
Transcript ID List	ENST00000245812.8; ENST00000599849.1; ENST00000596657.1
External Link	RMBase: m6A_site_414172
mod ID: M6ASITE038786		Click to Show/Hide the Full List
mod site	chr19:6375030-6375031:+		[6]
Sequence	GTTGGGGAATGGACAGCCTAACTGGGACATTGCAGTGGCTG
Motif Score	2.590089286
Cell/Tissue List	A549
Seq Type List	m6A-CLIP/IP
Transcript ID List	ENST00000245812.8; ENST00000599849.1
External Link	RMBase: m6A_site_414173
mod ID: M6ASITE038787		Click to Show/Hide the Full List
mod site	chr19:6375036-6375037:+		[2]
Sequence	GAATGGACAGCCTAACTGGGACATTGCAGTGGCTGCTTGCT
Motif Score	3.643047619
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; H1A; H1B; hESCs; MM6; Huh7; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; TIME; endometrial; HEC-1-A
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: m6A_site_414174
mod ID: M6ASITE038788		Click to Show/Hide the Full List
mod site	chr19:6375078-6375079:+		[2]
Sequence	GGGCCGGGATTTGCAGGGGAACCCAGGATGGCACTGGCCCA
Motif Score	2.930744048
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; H1A; H1B; hNPCs; hESCs; GM12878; MM6; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: m6A_site_414175
mod ID: M6ASITE038789		Click to Show/Hide the Full List
mod site	chr19:6375126-6375127:+		[2]
Sequence	CTCCAGGTGTGGCTGGCTGGACACATGGTCAAAGTCACAAG
Motif Score	3.643047619
Cell/Tissue List	HeLa; HepG2; HEK293T; A549; H1A; H1B; hNPCs; hESCs; GM12878; MM6; Jurkat; CD4T; peripheral-blood; GSC-11; HEK293A-TOA; iSLK; MSC; TIME; TREX; endometrial
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000245812.8
External Link	RMBase: m6A_site_414176

Pseudouridine (Pseudo)

In total 1 m6A sequence/site(s) in this target gene
mod ID: PSESITE000119		Click to Show/Hide the Full List
mod site	chr19:6375167-6375168:+		[7]
Sequence	GCCGGGAGAGTGGTGTCCTTTATTGCACTCACTGCTGGTCG
Transcript ID List	ENST00000245812.8
External Link	RMBase: Pseudo_site_2416

Ref 1	5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader. Cell Res. 2017 May;27(5):606-625. doi: 10.1038/cr.2017.55. Epub 2017 Apr 18.
Ref 2	VIRMA mediates preferential m(6)A mRNA methylation in 3'UTR and near stop codon and associates with alternative polyadenylation. Cell Discov. 2018 Feb 27;4:10. doi: 10.1038/s41421-018-0019-0. eCollection 2018.
Ref 3	Deciphering the "m(6)A Code" via Antibody-Independent Quantitative Profiling. Cell. 2019 Jul 25;178(3):731-747.e16. doi: 10.1016/j.cell.2019.06.013. Epub 2019 Jun 27.
Ref 4	Single-base mapping of m(6)A by an antibody-independent method. Sci Adv. 2019 Jul 3;5(7):eaax0250. doi: 10.1126/sciadv.aax0250. eCollection 2019 Jul.
Ref 5	DART-seq: an antibody-free method for global m(6)A detection. Nat Methods. 2019 Dec;16(12):1275-1280. doi: 10.1038/s41592-019-0570-0. Epub 2019 Sep 23.
Ref 6	A majority of m6A residues are in the last exons, allowing the potential for 3' UTR regulation. Genes Dev. 2015 Oct 1;29(19):2037-53. doi: 10.1101/gad.269415.115. Epub 2015 Sep 24.
Ref 7	Chemical pulldown reveals dynamic pseudouridylation of the mammalian transcriptome. Nat Chem Biol. 2015 Aug;11(8):592-7. doi: 10.1038/nchembio.1836. Epub 2015 Jun 15.

Epigenetic Target Information

Cite M6AREG

Correspondence

Prof. Feng ZHU

Prof. Shuiping Liu

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