Epigenetic Target Information

General Information of the Epigenetic Target (ID: EPITAR00497)

Target Name	Leucine rich repeat containing G protein-coupled receptor 5 (LGR5)
Synonyms	HG38; FEX; GPR67; GPR49; G protein-coupled receptor 49 Click to Show/Hide
Gene Name	LGR5
Sequence	MDTSRLGVLLSLPVLLQLATGGSSPRSGVLLRGCPTHCHCEPDGRMLLRVDCSDLGLSELPSNLSVFTSYLDLSMNNISQLLPNPLPSLRFLEELRLAGNALTYIPKGAFTGLYSLKVLMLQNNQLRHVPTEALQNLRSLQSLRLDANHISYVPPSCFSGLHSLRHLWLDDNALTEIPVQAFRSLSALQAMTLALNKIHHIPDYAFGNLSSLVVLHLHNNRIHSLGKKCFDGLHSLETLDLNYNNLDEFPTAIRTLSNLKELGFHSNNIRSIPEKAFVGNPSLITIHFYDNPIQFVGRSAFQHLPELRTLTLNGASQITEFPDLTGTANLESLTLTGAQISSLPQTVCNQLPNLQVLDLSYNLLEDLPSFSVCQKLQKIDLRHNEIYEIKVDTFQQLLSLRSLNLAWNKIAIIHPNAFSTLPSLIKLDLSSNLLSSFPITGLHGLTHLKLTGNHALQSLISSENFPELKVIEMPYAYQCCAFGVCENAYKISNQWNKGDNSSMDDLHKKDAGMFQAQDERDLEDFLLDFEEDLKALHSVQCSPSPGPFKPCEHLLDGWLIRIGVWTIAVLALTCNALVTSTVFRSPLYISPIKLLIGVIAAVNMLTGVSSAVLAGVDAFTFGSFARHGAWWENGVGCHVIGFLSIFASESSVFLLTLAALERGFSVKYSAKFETKAPFSSLKVIILLCALLALTMAAVPLLGGSKYGASPLCLPLPFGEPSTMGYMVALILLNSLCFLMMTIAYTKLYCNLDKGDLENIWDCSMVKHIALLLFTNCILNCPVAFLSFSSLINLTFISPEVIKFILLVVVPLPACLNPLLYILFNPHFKEDLVSLRKQTYVWTRSKHPSLMSINSDDVEKQSCDSTQALVTFTSSSITYDLPPSSVPSPAYPVTESCHLSSVAFVPCL Click to Show/Hide
Family	G-protein coupled receptor 1 family
Function	Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. Click to Show/Hide
Gene ID	8549
HGNC ID	HGNC:4504
Ensembl Gene ID	ENSG00000139292
KEGG ID	hsa:8549
Chromosomal Location	12q21.1

RNA Modification Sequencing Data Associated with the Target (ID: EPITAR00497)

Leucine rich repeat containing G protein-coupled receptor 5 (LGR5)

Adenosine-to-Inosine editing (A-to-I)

In total 1 m6A sequence/site(s) in this target gene
mod ID: A2ISITE005675		Click to Show/Hide the Full List
mod site	chr12:71527600-71527601:+		[1]
Sequence	GGCGATAATACAAAATATTTATAAAGCAAACTTTTGAGGAA
Transcript ID List	ENST00000536515.5; ENST00000540815.2; ENST00000266674.10; ENST00000550851.5
External Link	RMBase: RNA-editing_site_30137

5-methylcytidine (m5C)

In total 1 m6A sequence/site(s) in this target gene
mod ID: M5CSITE000082		Click to Show/Hide the Full List
mod site	chr12:71535183-71535184:+		[2]
Sequence	AATTTGCGAAGCCTTCAATCCCTGTAAGTATAGTAGACATT
Seq Type List	Bisulfite-seq
Transcript ID List	ENST00000536515.5; ENST00000550851.5; ENST00000540815.2; ENST00000266674.10
External Link	RMBase: m5C_site_10639

N6-methyladenosine (m6A)

In total 18 m6A sequence/site(s) in this target gene
mod ID: M6ASITE014152		Click to Show/Hide the Full List
mod site	chr12:71440231-71440232:+		[3]
Sequence	CAGGATGTTGCTCAGGGTGGACTGCTCCGACCTGGGGCTCT
Motif Score	4.065041667
Cell/Tissue List	HepG2
Seq Type List	m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000550851.5; ENST00000266674.10; ENST00000536515.5
External Link	RMBase: m6A_site_198985
mod ID: M6ASITE014153		Click to Show/Hide the Full List
mod site	chr12:71504615-71504616:+		[3]
Sequence	GTCTGTTTTCCCCCCCAGAGACCTCAGTATGAACAACATCA
Motif Score	2.876744048
Cell/Tissue List	HepG2
Seq Type List	m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000550851.5; ENST00000536515.5; ENST00000266674.10
External Link	RMBase: m6A_site_198986
mod ID: M6ASITE014154		Click to Show/Hide the Full List
mod site	chr12:71504627-71504628:+		[3]
Sequence	CCCCAGAGACCTCAGTATGAACAACATCAGTCAGCTGCTCC
Motif Score	2.951386905
Cell/Tissue List	HepG2
Seq Type List	m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000550851.5; ENST00000536515.5; ENST00000266674.10
External Link	RMBase: m6A_site_198987
mod ID: M6ASITE014155		Click to Show/Hide the Full List
mod site	chr12:71583656-71583657:+		[4]
Sequence	GGACTTCTAGGCCCCTTCAAACCCTGTGAACACCTGCTTGA
Motif Score	2.185083333
Cell/Tissue List	HEK293T; endometrial
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000536515.5; ENST00000266674.10; ENST00000550851.5; ENST00000540815.2
External Link	RMBase: m6A_site_198989
mod ID: M6ASITE014157		Click to Show/Hide the Full List
mod site	chr12:71583665-71583666:+		[4]
Sequence	GGCCCCTTCAAACCCTGTGAACACCTGCTTGATGGCTGGCT
Motif Score	2.951386905
Cell/Tissue List	HEK293T; endometrial
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000266674.10; ENST00000536515.5; ENST00000550851.5
External Link	RMBase: m6A_site_198990
mod ID: M6ASITE014158		Click to Show/Hide the Full List
mod site	chr12:71583705-71583706:+		[4]
Sequence	TGATCAGAATTGGAGTGTGGACCATAGCAGTTCTGGCACTT
Motif Score	3.622404762
Cell/Tissue List	HEK293T; endometrial
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000266674.10; ENST00000536515.5; ENST00000550851.5
External Link	RMBase: m6A_site_198991
mod ID: M6ASITE014159		Click to Show/Hide the Full List
mod site	chr12:71583788-71583789:+		[4]
Sequence	CTGTACATTTCCCCCATTAAACTGTTAATTGGGGTCATCGC
Motif Score	2.627720238
Cell/Tissue List	HEK293T; endometrial
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000266674.10; ENST00000550851.5; ENST00000536515.5
External Link	RMBase: m6A_site_198992
mod ID: M6ASITE014160		Click to Show/Hide the Full List
mod site	chr12:71583817-71583818:+
Sequence	TGGGGTCATCGCAGCAGTGAACATGCTCACGGGAGTCTCCA
Motif Score	2.951386905
Cell/Tissue List	HEK293T
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000550851.5; ENST00000536515.5; ENST00000540815.2; ENST00000266674.10
External Link	RMBase: m6A_site_198993
mod ID: M6ASITE014161		Click to Show/Hide the Full List
mod site	chr12:71584291-71584292:+		[5]
Sequence	AGACCTGGAGAATATTTGGGACTGCTCTATGGTAAAACACA
Motif Score	4.065041667
Cell/Tissue List	Huh7
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000536515.5; ENST00000550851.5; ENST00000540815.2; ENST00000266674.10
External Link	RMBase: m6A_site_198994
mod ID: M6ASITE014162		Click to Show/Hide the Full List
mod site	chr12:71584307-71584308:+		[5]
Sequence	TGGGACTGCTCTATGGTAAAACACATTGCCCTGTTGCTCTT
Motif Score	2.20572619
Cell/Tissue List	Huh7
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000266674.10; ENST00000550851.5; ENST00000536515.5; ENST00000540815.2
External Link	RMBase: m6A_site_198995
mod ID: M6ASITE014163		Click to Show/Hide the Full List
mod site	chr12:71584345-71584346:+		[5]
Sequence	CTTCACCAACTGCATCCTAAACTGCCCTGTGGCTTTCTTGT
Motif Score	2.627720238
Cell/Tissue List	Huh7
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000266674.10; ENST00000550851.5; ENST00000540815.2; ENST00000536515.5
External Link	RMBase: m6A_site_198996
mod ID: M6ASITE014164		Click to Show/Hide the Full List
mod site	chr12:71584384-71584385:+		[5]
Sequence	GTCCTTCTCCTCTTTAATAAACCTTACATTTATCAGTCCTG
Motif Score	2.185083333
Cell/Tissue List	Huh7
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000536515.5; ENST00000540815.2; ENST00000266674.10; ENST00000550851.5
External Link	RMBase: m6A_site_198997
mod ID: M6ASITE014165		Click to Show/Hide the Full List
mod site	chr12:71584521-71584522:+		[6]
Sequence	TGGTGAGCCTGAGAAAGCAAACCTACGTCTGGACAAGATCA
Motif Score	2.185083333
Cell/Tissue List	HEK293T; Huh7
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000266674.10; ENST00000540815.2; ENST00000550851.5; ENST00000536515.5
External Link	RMBase: m6A_site_198998
mod ID: M6ASITE014166		Click to Show/Hide the Full List
mod site	chr12:71584533-71584534:+		[6]
Sequence	GAAAGCAAACCTACGTCTGGACAAGATCAAAACACCCAAGC
Motif Score	3.643047619
Cell/Tissue List	HEK293T; Huh7
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000536515.5; ENST00000550851.5; ENST00000266674.10
External Link	RMBase: m6A_site_198999
mod ID: M6ASITE014167		Click to Show/Hide the Full List
mod site	chr12:71584544-71584545:+		[6]
Sequence	TACGTCTGGACAAGATCAAAACACCCAAGCTTGATGTCAAT
Motif Score	2.20572619
Cell/Tissue List	HEK293T; Huh7
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000540815.2; ENST00000536515.5; ENST00000266674.10; ENST00000550851.5
External Link	RMBase: m6A_site_199000
mod ID: M6ASITE014168		Click to Show/Hide the Full List
mod site	chr12:71584586-71584587:+		[7]
Sequence	AACTCTGATGATGTCGAAAAACAGTCCTGTGACTCAACTCA
Motif Score	2.20572619
Cell/Tissue List	HEK293T; Huh7; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000266674.10; ENST00000550851.5; ENST00000540815.2; ENST00000536515.5
External Link	RMBase: m6A_site_199001
mod ID: M6ASITE014169		Click to Show/Hide the Full List
mod site	chr12:71584770-71584771:+		[5]
Sequence	AATGTTTTCAAAGGTTGAGAACCTGAAAATGTGAGATTGAG
Motif Score	2.930744048
Cell/Tissue List	Huh7
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000540815.2; ENST00000550851.5; ENST00000266674.10; ENST00000536515.5
External Link	RMBase: m6A_site_199002
mod ID: M6ASITE014170		Click to Show/Hide the Full List
mod site	chr12:71586304-71586305:+		[5]
Sequence	ATAAAATGTGTGACTATGAAACAAAATATTTTCAAGTCTAT
Motif Score	2.20572619
Cell/Tissue List	Huh7
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000266674.10; ENST00000550851.5
External Link	RMBase: m6A_site_199003

Ref 1	RADAR: a rigorously annotated database of A-to-I RNA editing. Nucleic Acids Res. 2014 Jan;42(Database issue):D109-13. doi: 10.1093/nar/gkt996. Epub 2013 Oct 25.
Ref 2	5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader. Cell Res. 2017 May;27(5):606-625. doi: 10.1038/cr.2017.55. Epub 2017 Apr 18.
Ref 3	[Action myoclonias and paranoid alternative psychosis in progressive myoclonic epilepsy (late form of the Lafora type)]. Nervenarzt. 1986 Apr;57(4):233-7.
Ref 4	m(6)A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer. Nat Cell Biol. 2018 Sep;20(9):1074-1083. doi: 10.1038/s41556-018-0174-4. Epub 2018 Aug 27.
Ref 5	N6-Methyladenosine in Flaviviridae Viral RNA Genomes Regulates Infection. Cell Host Microbe. 2016 Nov 9;20(5):654-665. doi: 10.1016/j.chom.2016.09.015. Epub 2016 Oct 20.
Ref 6	Perturbation of m6A writers reveals two distinct classes of mRNA methylation at internal and 5' sites. Cell Rep. 2014 Jul 10;8(1):284-96. doi: 10.1016/j.celrep.2014.05.048. Epub 2014 Jun 26.
Ref 7	Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons. Cell. 2012 Jun 22;149(7):1635-46. doi: 10.1016/j.cell.2012.05.003. Epub 2012 May 17.

Epigenetic Target Information

Cite M6AREG

Correspondence

Prof. Feng ZHU

Prof. Shuiping Liu

Visitor Map