Epigenetic Target Information

General Information of the Epigenetic Target (ID: EPITAR00493)

Target Name	GATA binding protein 4 (GATA4)
Synonyms	GATA-binding protein 4 Click to Show/Hide
Gene Name	GATA4
Sequence	MYQSLAMAANHGPPPGAYEAGGPGAFMHGAGAASSPVYVPTPRVPSSVLGLSYLQGGGAGSASGGASGGSSGGAASGAGPGTQQGSPGWSQAGADGAAYTPPPVSPRFSFPGTTGSLAAAAAAAAAREAAAYSSGGGAAGAGLAGREQYGRAGFAGSYSSPYPAYMADVGASWAAAAAASAGPFDSPVLHSLPGRANPAARHPNLDMFDDFSEGRECVNCGAMSTPLWRRDGTGHYLCNACGLYHKMNGINRPLIKPQRRLSASRRVGLSCANCQTTTTTLWRRNAEGEPVCNACGLYMKLHGVPRPLAMRKEGIQTRKRKPKNLNKSKTPAAPSGSESLPPASGASSNSSNATTSSSEEMRPIKTEPGLSSHYGHSSSVSQTFSVSAMSGHGPSIHPVLSALKLSPQGYASPVSQSPQTSSKQDSWNSLVLADSHGDIITA Click to Show/Hide
Function	Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development and function (PubMed:24000169, PubMed:27984724, PubMed:35182466). In cooperation with TBX5, it binds to cardiac super-enhancers and promotes cardiomyocyte gene expression, while it down-regulates endocardial and endothelial gene expression (PubMed:27984724). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression. Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, sphingosine-1-phosphate lyase (PubMed:15734735). Click to Show/Hide
Gene ID	2626
HGNC ID	HGNC:4173
Ensembl Gene ID	ENSG00000136574
KEGG ID	hsa:2626
Chromosomal Location	8p23.1

RNA Modification Sequencing Data Associated with the Target (ID: EPITAR00493)

GATA binding protein 4 (GATA4)

5-methylcytidine (m5C)

In total 1 m6A sequence/site(s) in this target gene
mod ID: M5CSITE004254		Click to Show/Hide the Full List
mod site	chr8:11676967-11676968:+		[1]
Sequence	AGACACGGCTCACAACGTCTCCCTCCCACCCGGCTGAGAAC
Seq Type List	Bisulfite-seq
Transcript ID List	ENST00000532977.1; ENST00000528712.5
External Link	RMBase: m5C_site_41031

N6-methyladenosine (m6A)

In total 38 m6A sequence/site(s) in this target gene
mod ID: M6ASITE083685		Click to Show/Hide the Full List
mod site	chr8:11708202-11708203:+		[2]
Sequence	TTTGATTTTTGATCTTCGCGACAGTTCCTCCCACGCATATT
Motif Score	2.865571429
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000526974.1; ENST00000528712.5; ENST00000335135.8; ENST00000528027.1; ENST00000532977.1; ENST00000526716.5
External Link	RMBase: m6A_site_786870
mod ID: M6ASITE083686		Click to Show/Hide the Full List
mod site	chr8:11708607-11708608:+		[3]
Sequence	GGGAGCCGACGGAGCCGCTTACACCCCGCCGCCGGTGTCGC
Motif Score	2.07285119
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528027.1; ENST00000528712.5; ENST00000335135.8; ENST00000526716.5; ENST00000532059.5; ENST00000622443.2
External Link	RMBase: m6A_site_786871
mod ID: M6ASITE083687		Click to Show/Hide the Full List
mod site	chr8:11708805-11708806:+		[3]
Sequence	CTCCAGCCCCTACCCGGCTTACATGGCCGACGTGGGCGCGT
Motif Score	2.07285119
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000532059.5; ENST00000335135.8; ENST00000528712.5; ENST00000622443.2; ENST00000526716.5
External Link	RMBase: m6A_site_786872
mod ID: M6ASITE083688		Click to Show/Hide the Full List
mod site	chr8:11708914-11708915:+		[3]
Sequence	CGGGCCAACCCGGCCGCCCGACACCCCAATCTCGGTGAGTA
Motif Score	2.865571429
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000622443.2; ENST00000528712.5; ENST00000532059.5; ENST00000526716.5; ENST00000335135.8
External Link	RMBase: m6A_site_786873
mod ID: M6ASITE083689		Click to Show/Hide the Full List
mod site	chr8:11749035-11749036:+		[3]
Sequence	CAACGCCTGCGGCCTCTACCACAAGATGAACGGCATCAACC
Motif Score	2.053113095
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000526716.5; ENST00000622443.2; ENST00000528712.5; ENST00000335135.8; ENST00000532059.5
External Link	RMBase: m6A_site_786874
mod ID: M6ASITE083690		Click to Show/Hide the Full List
mod site	chr8:11750219-11750220:+		[3]
Sequence	GTGCAATGCCTGCGGCCTCTACATGAAGCTCCACGGGGTAC
Motif Score	2.078666667
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000335135.8; ENST00000528712.5; ENST00000622443.2; ENST00000526716.5; ENST00000532059.5
External Link	RMBase: m6A_site_786875
mod ID: M6ASITE083691		Click to Show/Hide the Full List
mod site	chr8:11758504-11758505:+		[2]
Sequence	TTCCCTCCTCAAATTCCTGCACGGACCTGGGACTTGGAGGA
Motif Score	2.80452381
Cell/Tissue List	HEK293T
Seq Type List	DART-seq
Transcript ID List	ENST00000335135.8; ENST00000532059.5; ENST00000526021.1; ENST00000528712.5; ENST00000622443.2
External Link	RMBase: m6A_site_786876
mod ID: M6ASITE083692		Click to Show/Hide the Full List
mod site	chr8:11758616-11758617:+		[3]
Sequence	TGGGAAGAAACTTGAAGTCGACAATCTGGTTAGGGGAAGCG
Motif Score	2.865571429
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000622443.2; ENST00000335135.8; ENST00000528712.5; ENST00000526021.1
External Link	RMBase: m6A_site_786877
mod ID: M6ASITE083693		Click to Show/Hide the Full List
mod site	chr8:11758663-11758664:+		[3]
Sequence	GGATTTTCTCAGATGCCTTTACACGCTGATGGGACTGGAGG
Motif Score	2.07285119
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000335135.8; ENST00000622443.2; ENST00000528712.5; ENST00000526021.1
External Link	RMBase: m6A_site_786878
mod ID: M6ASITE083694		Click to Show/Hide the Full List
mod site	chr8:11758706-11758707:+		[3]
Sequence	GCCCACCCTTCAGCACGAGCACACTGCATCTCTCCTGTGAG
Motif Score	2.830589286
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000335135.8; ENST00000528712.5; ENST00000622443.2; ENST00000526021.1
External Link	RMBase: m6A_site_786879
mod ID: M6ASITE083696		Click to Show/Hide the Full List
mod site	chr8:11758826-11758827:+		[4]
Sequence	CTAGCACCGAGGATCTGAGAACAAGCGGAGGGCCGGGCCCT
Motif Score	2.951386905
Cell/Tissue List	HepG2; hESC-HEK293T
Seq Type List	m6A-seq; MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000622443.2; ENST00000335135.8
External Link	RMBase: m6A_site_786880
mod ID: M6ASITE083697		Click to Show/Hide the Full List
mod site	chr8:11758850-11758851:+		[5]
Sequence	GCGGAGGGCCGGGCCCTGGGACCCCTGCTCCAGCCCGAATG
Motif Score	3.622404762
Cell/Tissue List	HepG2
Seq Type List	m6A-seq
Transcript ID List	ENST00000622443.2; ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786881
mod ID: M6ASITE083698		Click to Show/Hide the Full List
mod site	chr8:11758916-11758917:+		[5]
Sequence	GATGCGACGGGCCCCTGGGGACAGGCCCTTGCCCCATCCAT
Motif Score	3.643047619
Cell/Tissue List	HepG2
Seq Type List	m6A-seq
Transcript ID List	ENST00000335135.8; ENST00000622443.2; ENST00000528712.5
External Link	RMBase: m6A_site_786882
mod ID: M6ASITE083699		Click to Show/Hide the Full List
mod site	chr8:11759019-11759020:+		[5]
Sequence	TGTGACATACAAGTGACTGAACACTTCCTGGGGAGCTACAG
Motif Score	2.951386905
Cell/Tissue List	HepG2
Seq Type List	m6A-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8; ENST00000622443.2
External Link	RMBase: m6A_site_786883
mod ID: M6ASITE083700		Click to Show/Hide the Full List
mod site	chr8:11759141-11759142:+		[3]
Sequence	CCTTTGCTCCTTCACTTCCAACATCTCTCAAAATAAAAATC
Motif Score	2.173910714
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786884
mod ID: M6ASITE083701		Click to Show/Hide the Full List
mod site	chr8:11759204-11759205:+		[3]
Sequence	CAGCTCTGCCCGCAGCTTGTACATGTCTCTCCCCTGGCAAA
Motif Score	2.856142857
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786885
mod ID: M6ASITE083702		Click to Show/Hide the Full List
mod site	chr8:11759225-11759226:+		[3]
Sequence	CATGTCTCTCCCCTGGCAAAACAAGAGCTGGGTAGTTTAGC
Motif Score	2.20572619
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786886
mod ID: M6ASITE083703		Click to Show/Hide the Full List
mod site	chr8:11759294-11759295:+		[3]
Sequence	GACCCTTCGTTCACCGTGTCACACATAGAGGGGTTCTGAGT
Motif Score	2.047297619
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786887
mod ID: M6ASITE083704		Click to Show/Hide the Full List
mod site	chr8:11759626-11759627:+		[3]
Sequence	GAAAGAAGACGACTGCTAAGACACGGCAGGGGGGCCTGGAG
Motif Score	2.897386905
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000335135.8; ENST00000528712.5
External Link	RMBase: m6A_site_786888
mod ID: M6ASITE083705		Click to Show/Hide the Full List
mod site	chr8:11759696-11759697:+		[3]
Sequence	TCTGCCGCGTTCTCCTCTGCACATTGCTGTTTCTGCCCCTG
Motif Score	2.830589286
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786889
mod ID: M6ASITE083707		Click to Show/Hide the Full List
mod site	chr8:11759792-11759793:+		[3]
Sequence	TGGCATTACTACGCCTCCCCACACGCCCAGACCCCTCACTC
Motif Score	2.053113095
Cell/Tissue List	hESC-HEK293T
Seq Type List	MAZTER-seq
Transcript ID List	ENST00000528712.5; ENST00000335135.8
External Link	RMBase: m6A_site_786890
mod ID: M6ASITE094771		Click to Show/Hide the Full List
mod site	KZ208915.1:1588580-1588581:-
Sequence	GGCAGCCAGAGTCCCTCAGGACCTGCAGCCTCGCCCCGGCA
Motif Score	3.622404762
Cell/Tissue List	HEK293T
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000646696.1; ENST00000647274.1
External Link	RMBase: m6A_site_882281
mod ID: M6ASITE094772		Click to Show/Hide the Full List
mod site	KZ208915.1:1588683-1588684:-		[6]
Sequence	TCTGGGGAGAGTGTAAGTGGACAGAGTCCTGGTCAGGGGGC
Motif Score	3.643047619
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882282
mod ID: M6ASITE094773		Click to Show/Hide the Full List
mod site	KZ208915.1:1588757-1588758:-		[6]
Sequence	GGGTTCTGAGTAAGAACAAAACATTCTGCTGCTCAAGCCAG
Motif Score	2.20572619
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882283
mod ID: M6ASITE094774		Click to Show/Hide the Full List
mod site	KZ208915.1:1588762-1588763:-		[6]
Sequence	TAGAGGGGTTCTGAGTAAGAACAAAACATTCTGCTGCTCAA
Motif Score	2.951386905
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000646696.1; ENST00000647274.1
External Link	RMBase: m6A_site_882284
mod ID: M6ASITE094775		Click to Show/Hide the Full List
mod site	KZ208915.1:1588806-1588807:-		[6]
Sequence	CCCCCTCGAGTTCACTGCAGACCCTTCGTTCACCGTGTCAC
Motif Score	2.876744048
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882285
mod ID: M6ASITE094776		Click to Show/Hide the Full List
mod site	KZ208915.1:1588856-1588857:-		[6]
Sequence	CATGTCTCTCCCCTGGCAAAACAAGAGCTGGGTAGTTTAGC
Motif Score	2.20572619
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882286
mod ID: M6ASITE094777		Click to Show/Hide the Full List
mod site	KZ208915.1:1589062-1589063:-		[7]
Sequence	TGTGACACACAAGTGACTGAACACTTCCTGGGGAGCTACAG
Motif Score	2.951386905
Cell/Tissue List	HepG2; HEK293T; U2OS; H1A; A549; Huh7; HEK293A-TOA
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000646696.1; ENST00000645330.1; ENST00000647274.1
External Link	RMBase: m6A_site_882287
mod ID: M6ASITE094778		Click to Show/Hide the Full List
mod site	KZ208915.1:1589091-1589092:-		[4]
Sequence	ATACCAAATCTGACTCCAAAACTGTGGGGTGTGACACACAA
Motif Score	2.627720238
Cell/Tissue List	HepG2; HEK293T; A549; U2OS; H1A; Huh7; HEK293A-TOA
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000645330.1; ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882288
mod ID: M6ASITE094779		Click to Show/Hide the Full List
mod site	KZ208915.1:1589165-1589166:-		[7]
Sequence	GATGTGACGGGCCCCTGGGGACAGGCCCTTGCCCCATCCAT
Motif Score	3.643047619
Cell/Tissue List	HepG2; HEK293T; HEK293A-TOA
Seq Type List	m6A-seq; MeRIP-seq
Transcript ID List	ENST00000646696.1; ENST00000647274.1; ENST00000645330.1
External Link	RMBase: m6A_site_882289
mod ID: M6ASITE094780		Click to Show/Hide the Full List
mod site	KZ208915.1:1589231-1589232:-		[8]
Sequence	GCGGAGGGCCGGGCCCTGGGACCCCTGCTCCAGCCCGAATG
Motif Score	3.622404762
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000646696.1; ENST00000645330.1; ENST00000647274.1
External Link	RMBase: m6A_site_882290
mod ID: M6ASITE094781		Click to Show/Hide the Full List
mod site	KZ208915.1:1589255-1589256:-		[6]
Sequence	CTAGCACCGAGGATCTGAGAACAAGCGGAGGGCCGGGCCCT
Motif Score	2.951386905
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000645330.1; ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882291
mod ID: M6ASITE094782		Click to Show/Hide the Full List
mod site	KZ208915.1:1589298-1589299:-		[6]
Sequence	CGTTCCCCACTGTGGCCTAGACCGTGGGTTTTGCATTGTGT
Motif Score	2.876744048
Cell/Tissue List	HEK293T; HEK293A-TOA
Seq Type List	MeRIP-seq; m6A-seq
Transcript ID List	ENST00000647274.1; ENST00000645330.1; ENST00000646696.1
External Link	RMBase: m6A_site_882292
mod ID: M6ASITE094783		Click to Show/Hide the Full List
mod site	KZ208915.1:1589348-1589349:-
Sequence	ATCTCTCCTGTGAGTTGGAGACTTCTTTCCCAAGATGTCCT
Motif Score	3.319380952
Cell/Tissue List	HEK293T
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000645330.1; ENST00000647218.1; ENST00000647274.1; ENST00000646696.1
External Link	RMBase: m6A_site_882293
mod ID: M6ASITE094784		Click to Show/Hide the Full List
mod site	KZ208915.1:1589405-1589406:-
Sequence	TGCCTTTACACGCTGATGGGACTGGAGGGAGCCCACCCTTC
Motif Score	4.065041667
Cell/Tissue List	HEK293T
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000646696.1; ENST00000645330.1; ENST00000647274.1; ENST00000647218.1
External Link	RMBase: m6A_site_882294
mod ID: M6ASITE094785		Click to Show/Hide the Full List
mod site	KZ208915.1:1589476-1589477:-
Sequence	AGAACAACAACTGGGAAGAAACTTGAAGTCGACAATCTGGT
Motif Score	2.627720238
Cell/Tissue List	HEK293T
Seq Type List	MeRIP-seq
Transcript ID List	ENST00000645330.1; ENST00000646696.1; ENST00000647218.1; ENST00000647274.1
External Link	RMBase: m6A_site_882295
mod ID: M6ASITE094786		Click to Show/Hide the Full List
mod site	KZ208915.1:1647455-1647456:-		[9]
Sequence	GCAGGGAAGGAGTATCGCAGACCGGCGCCCAGGCCCAGCGG
Motif Score	2.876744048
Cell/Tissue List	HEK293T
Seq Type List	m6A-seq
Transcript ID List	ENST00000644275.1; ENST00000646696.1; ENST00000642753.1
External Link	RMBase: m6A_site_882296
mod ID: M6ASITE094787		Click to Show/Hide the Full List
mod site	KZ208915.1:1647487-1647488:-		[9]
Sequence	GGCTGCCCGTGCGGGTGAGGACCGAGTGCCGCGCAGGGAAG
Motif Score	3.622404762
Cell/Tissue List	HEK293T
Seq Type List	m6A-seq
Transcript ID List	ENST00000646696.1; ENST00000642753.1; ENST00000644275.1
External Link	RMBase: m6A_site_882297

Ref 1	5-methylcytosine promotes mRNA export - NSUN2 as the methyltransferase and ALYREF as an m(5)C reader. Cell Res. 2017 May;27(5):606-625. doi: 10.1038/cr.2017.55. Epub 2017 Apr 18.
Ref 2	DART-seq: an antibody-free method for global m(6)A detection. Nat Methods. 2019 Dec;16(12):1275-1280. doi: 10.1038/s41592-019-0570-0. Epub 2019 Sep 23.
Ref 3	Deciphering the "m(6)A Code" via Antibody-Independent Quantitative Profiling. Cell. 2019 Jul 25;178(3):731-747.e16. doi: 10.1016/j.cell.2019.06.013. Epub 2019 Jun 27.
Ref 4	Histone H3 trimethylation at lysine 36 guides m(6)A RNA modification co-transcriptionally. Nature. 2019 Mar;567(7748):414-419. doi: 10.1038/s41586-019-1016-7. Epub 2019 Mar 13.
Ref 5	[Action myoclonias and paranoid alternative psychosis in progressive myoclonic epilepsy (late form of the Lafora type)]. Nervenarzt. 1986 Apr;57(4):233-7.
Ref 6	The U6 snRNA m(6)A Methyltransferase METTL16 Regulates SAM Synthetase Intron Retention. Cell. 2017 May 18;169(5):824-835.e14. doi: 10.1016/j.cell.2017.05.003.
Ref 7	Efficacy of MEK inhibition in patients with histiocytic neoplasms. Nature. 2019 Mar;567(7749):521-524. doi: 10.1038/s41586-019-1012-y. Epub 2019 Mar 13.
Ref 8	Comprehensive analysis of mRNA methylation reveals enrichment in 3' UTRs and near stop codons. Cell. 2012 Jun 22;149(7):1635-46. doi: 10.1016/j.cell.2012.05.003. Epub 2012 May 17.
Ref 9	Dynamics of Human and Viral RNA Methylation during Zika Virus Infection. Cell Host Microbe. 2016 Nov 9;20(5):666-673. doi: 10.1016/j.chom.2016.10.002. Epub 2016 Oct 20.

Epigenetic Target Information

Cite M6AREG

Correspondence

Prof. Feng ZHU

Prof. Shuiping Liu

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