m6A-centered Crosstalk Information
Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation
| Crosstalk ID |
M6ACROT05667
|
[1] | |||
m6A modification
CARMN
CARMN
YTHDF2
 : m6A sites
Direct
Inhibition
Non-coding RNA
CARMN
miR-21-5p
lncRNA miRNA circRNA
|
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| m6A Modification: | |||||
|---|---|---|---|---|---|
| m6A Regulator | YTH domain-containing family protein 2 (YTHDF2) | READER | |||
| m6A Target | Cardiac mesoderm enhancer-associated non-coding RNA (CARMN) | ||||
| Epigenetic Regulation that have Cross-talk with This m6A Modification: | |||||
| Epigenetic Regulation Type | Non-coding RNA (ncRNA) | ||||
| Epigenetic Regulator | Cardiac mesoderm enhancer-associated non-coding RNA (CARMN) | LncRNA | View Details | ||
| Regulated Target | hsa-miR-21-5p | View Details | |||
| Crosstalk Relationship | m6A → ncRNA | Inhibition | |||
| Crosstalk Mechanism | m6A regulators directly modulate the functionality of ncRNAs through specific targeting ncRNA | ||||
| Crosstalk Summary | m6A reader YTHDF2 could recognize m6A-modified Cardiac mesoderm enhancer-associated non-coding RNA (CARMN) and promote its degradation in CC cells, thereby affecting the expression of its downstream target hsa-miR-21-5p. | ||||
| Responsed Disease | Cervical cancer | ICD-11: 2C77 | |||
| Cell Process | mRNA stability | ||||
In-vitro Model |
HeLa | Endocervical adenocarcinoma | Homo sapiens | CVCL_0030 | |
| SiHa | Cervical squamous cell carcinoma | Homo sapiens | CVCL_0032 | ||
| In-vivo Model | After approval by Ethics Committee of School of Public Health, Southeast University, BALB/c nude mice (4-5 weeks old) were purchased and raised in SPF animal house. 1 × 107 HeLa cells or NC cells with stable overexpression of CARMN were prepared in each nude mouse. The mice were killed 3 weeks later, and tumor volume was measured. | ||||