m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00678				[1], [2], [3], [4]
RNA modification MALAT1 MALAT1 FTO Demethylation : modification sites Indirect Inhibition m⁶A modification hsa-mir-181b-1 hsa-mir-181b-1 ALKBH5 Demethylation : m⁶A sites
m6A Regulator	RNA demethylase ALKBH5 (ALKBH5)			ERASER	Regulator Info
m6A Target	hsa-mir-181b-1				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> N6,2'-O-dimethyladenosine (m6Am)
Epigenetic Regulator	Fat mass and obesity-associated protein (FTO)			ERASER	View Details
Regulated Target	Metastasis associated lung adenocarcinoma transcript 1 (MALAT1)				View Details
Crosstalk Relationship	m6Am → m6A			Inhibition
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	FTO interacts with Metastasis associated lung adenocarcinoma transcript 1 (MALAT1), decreasing it's m6Am level and inhibiting its physical interaction with hsa-mir-181b-1, which was regulated by ALKBH5-mediated m6A modification.
In-vitro Model	253J	Bladder carcinoma	Homo sapiens		CVCL_7935
	HT-1197	Recurrent bladder carcinoma	Homo sapiens		CVCL_1291
	HT-1376	Bladder carcinoma	Homo sapiens		CVCL_1292
	U2OS	Osteosarcoma	Homo sapiens		CVCL_0042

References

Ref 1	FTO modifies the m6A level of MALAT and promotes bladder cancer progression. Clin Transl Med. 2021 Feb;11(2):e310. doi: 10.1002/ctm2.310.
Ref 2	The tumor-suppressive effects of alpha-ketoglutarate-dependent dioxygenase FTO via N6-methyladenosine RNA methylation on bladder cancer patients. Bioengineered. 2021 Dec;12(1):5323-5333. doi: 10.1080/21655979.2021.1964893.
Ref 3	ALKBH5 suppresses tumor progression via an m(6)A-dependent epigenetic silencing of pre-miR-181b-1/YAP signaling axis in osteosarcoma. Cell Death Dis. 2021 Jan 11;12(1):60. doi: 10.1038/s41419-020-03315-x.
Ref 4	LncRNA MALAT1 Suppression Protects Endothelium against oxLDL-Induced Inflammation via Inhibiting Expression of MiR-181b Target Gene TOX. Oxid Med Cell Longev. 2019 Dec 14;2019:8245810. doi: 10.1155/2019/8245810. eCollection 2019.