m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00630				[1], [2], [3]
m⁶A modification hsa-mir-146a hsa-mir-146a METTL14 Methylation : m⁶A sites Indirect Enhancement RNA modification BRCA1 BRCA1 ALYREF : modification sites
m6A Regulator	Methyltransferase-like 14 (METTL14)			WRITER	Regulator Info
m6A Target	hsa-mir-146a				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	THO complex subunit 4 (ALYREF)			READER	View Details
Regulated Target	Breast cancer type 1 susceptibility protein (BRCA1)				View Details
Crosstalk Relationship	m6A → m5C			Enhancement
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL14 interacts with hsa-mir-146a, reading its m6A level and promoting its physical interaction with Breast cancer type 1 susceptibility protein (BRCA1), which was regulated by ALYREF-mediated m5C modification.
In-vitro Model	HeLa	Endocervical adenocarcinoma	Homo sapiens		CVCL_0030
	MDA-MB-231	Breast adenocarcinoma	Homo sapiens		CVCL_0062
	MCF-7	Invasive breast carcinoma	Homo sapiens		CVCL_0031

References

Ref 1	Suppression of DDX39B sensitizes ovarian cancer cells to DNA-damaging chemotherapeutic agents via destabilizing BRCA1 mRNA. Oncogene. 2020 Nov;39(47):7051-7062. doi: 10.1038/s41388-020-01482-x. Epub 2020 Sep 28.
Ref 2	METTL14 promotes the migration and invasion of breast cancer cells by modulating N6?methyladenosine and hsa?miR?146a?5p expression. Oncol Rep. 2020 May;43(5):1375-1386. doi: 10.3892/or.2020.7515. Epub 2020 Feb 24.
Ref 3	Targeting MALAT1 induces DNA damage and sensitize non-small cell lung cancer cells to cisplatin by repressing BRCA1. Cancer Chemother Pharmacol. 2020 Nov;86(5):663-672. doi: 10.1007/s00280-020-04152-7. Epub 2020 Oct 8.