m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00629				[1], [2], [3], [4]
RNA modification GAS5 GAS5 YBX1 : modification sites Indirect Enhancement m⁶A modification hsa-mir-146a hsa-mir-146a METTL14 Methylation : m⁶A sites
m6A Regulator	Methyltransferase-like 14 (METTL14)			WRITER	Regulator Info
m6A Target	hsa-mir-146a				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> 5-methylcytidine (m5C)
Epigenetic Regulator	Y-box-binding protein 1 (YBX1)			READER	View Details
Regulated Target	Growth arrest specific 5 (GAS5)				View Details
Crosstalk Relationship	m5C → m6A			Enhancement
Crosstalk Mechanism	RNA modification indirectly impacts m6A modification through downstream signaling pathways
Crosstalk Summary	YBX1 interacts with Growth arrest specific 5 (GAS5), reading its m5C sites and promoting its physical interaction with hsa-mir-146a, which was regulated by METTL14-mediated m6A modification.
In-vitro Model	HGC-27	Gastric carcinoma	Homo sapiens		CVCL_1279
	MDA-MB-231	Breast adenocarcinoma	Homo sapiens		CVCL_0062
	MCF-7	Invasive breast carcinoma	Homo sapiens		CVCL_0031

References

Ref 1	lncRNA GAS5 enhances G1 cell cycle arrest via binding to YBX1 to regulate p21 expression in stomach cancer. Sci Rep. 2015 May 11;5:10159. doi: 10.1038/srep10159.
Ref 2	Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis. Theranostics. 2019 Jul 28;9(19):5558-5576. doi: 10.7150/thno.34463. eCollection 2019.
Ref 3	METTL14 promotes the migration and invasion of breast cancer cells by modulating N6?methyladenosine and hsa?miR?146a?5p expression. Oncol Rep. 2020 May;43(5):1375-1386. doi: 10.3892/or.2020.7515. Epub 2020 Feb 24.
Ref 4	LncRNA GAS5 upregulates Smad4 to suppress the apoptosis of chondrocytes induced by lipopolysaccharide. Arch Gerontol Geriatr. 2021 Nov-Dec;97:104478. doi: 10.1016/j.archger.2021.104478. Epub 2021 Jul 23.