m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00516				[1], [2], [3], [4]
m⁶A modification GAS5 GAS5 YTHDF3 : m⁶A sites Indirect Inhibition RNA modification MIR221 MIR221 ADARB1 Methylation : modification sites
m6A Regulator	YTH domain-containing family protein 3 (YTHDF3)			READER	Regulator Info
m6A Target	Growth arrest specific 5 (GAS5)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Double-stranded RNA-specific editase 1 (ADARB1)			WRITER	View Details
Regulated Target	MicroRNA 221 (MIR221)				View Details
Crosstalk Relationship	m6A → A-to-I			Inhibition
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	YTHDF3 interacts with Growth arrest specific 5 (GAS5), increasing its m6A level and inhibiting its physical interaction with MicroRNA 221 (MIR221), which was regulated by ADARB1-mediated A-to-I modification.
In-vitro Model	HCT 116	Colon carcinoma	Homo sapiens		CVCL_0291
	RKO	Colon carcinoma	Homo sapiens		CVCL_0504
	U-118MG	Astrocytoma	Homo sapiens		CVCL_0633

References

Ref 1	Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m(6)A reader YTHDF3. Mol Cancer. 2019 Oct 16;18(1):143. doi: 10.1186/s12943-019-1079-y.
Ref 2	Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. Genome Biol. 2015 Jan 13;16(1):5. doi: 10.1186/s13059-014-0575-z.
Ref 3	Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression. Aging (Albany NY). 2019 Oct 20;11(20):8745-8759. doi: 10.18632/aging.102249. Epub 2019 Oct 20.
Ref 4	LncRNAGAS5 sponges miRNA-221 to promote neurons apoptosis by up-regulated PUMA under hypoxia condition. Neurol Res. 2020 Jan;42(1):8-16. doi: 10.1080/01616412.2019.1672382. Epub 2019 Dec 27.