m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00440				[1], [2], [3]
m⁶A modification NEAT1 NEAT1 METTL14 Methylation : m⁶A sites Indirect Inhibition RNA modification MIR214 MIR214 ADARB1 Methylation : modification sites
m6A Regulator	Methyltransferase-like 14 (METTL14)			WRITER	Regulator Info
m6A Target	Nuclear paraspeckle assembly transcript 1 (NEAT1)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Double-stranded RNA-specific editase 1 (ADARB1)			WRITER	View Details
Regulated Target	MicroRNA 214 (MIR214)				View Details
Crosstalk Relationship	m6A → A-to-I			Inhibition
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	METTL14 interacts with Nuclear paraspeckle assembly transcript 1 (NEAT1), increasing its m6A level and inhibiting its physical interaction with MicroRNA 214 (MIR214), which was regulated by ADARB1-mediated A-to-I modification.
In-vitro Model	769-P	Renal cell carcinoma	Homo sapiens		CVCL_1050
	786-O	Renal cell carcinoma	Homo sapiens		CVCL_1051
	Huh-7	Adult hepatocellular carcinoma	Homo sapiens		CVCL_0336

References

Ref 1	Methyltransferase-like 14 suppresses growth and metastasis of renal cell carcinoma by decreasing long noncoding RNA NEAT1. Cancer Sci. 2022 Feb;113(2):446-458. doi: 10.1111/cas.15212. Epub 2021 Dec 16.
Ref 2	ADAR2-mediated editing of miR-214 and miR-122 precursor and antisense RNA transcripts in liver cancers. PLoS One. 2013 Dec 27;8(12):e81922. doi: 10.1371/journal.pone.0081922. eCollection 2013.
Ref 3	LncRNA NEAT1 sponges miR-214 to regulate M2 macrophage polarization by regulation of B7-H3 in multiple myeloma. Mol Immunol. 2020 Jan;117:20-28. doi: 10.1016/j.molimm.2019.10.026. Epub 2019 Nov 12.