m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00434				[1], [2], [3], [4], [5]
m⁶A modification NEAT1 NEAT1 ALKBH5 Demethylation : m⁶A sites Indirect Enhancement RNA modification MIR125A MIR125A METTL1 Methylation : modification sites
m6A Regulator	RNA demethylase ALKBH5 (ALKBH5)			ERASER	Regulator Info
m6A Target	Nuclear paraspeckle assembly transcript 1 (NEAT1)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> N7-methylguanosine (m7G)
Epigenetic Regulator	Methyltransferase-like protein 1 (METTL1)			WRITER	View Details
Regulated Target	MicroRNA 125a (MIR125A)				View Details
Crosstalk Relationship	m6A → m7G			Enhancement
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	ALKBH5 interacts with Nuclear paraspeckle assembly transcript 1 (NEAT1), increasing its m6A level and promoting its physical interaction with MicroRNA 125a (MIR125A), which was regulated by METTL1-mediated m7G modification.
In-vitro Model	BGC-823	Gastric carcinoma	Homo sapiens		CVCL_3360
	SGC-7901	Gastric carcinoma	Homo sapiens		CVCL_0520
	SW620	Colon adenocarcinoma	Homo sapiens		CVCL_0547
	RKO	Colon carcinoma	Homo sapiens		CVCL_0504
	A-549	Lung adenocarcinoma	Homo sapiens		CVCL_0023

References

Ref 1	ALKBH5 promotes invasion and metastasis of gastric cancer by decreasing methylation of the lncRNA NEAT1. J Physiol Biochem. 2019 Aug;75(3):379-389. doi: 10.1007/s13105-019-00690-8. Epub 2019 Jul 9.
Ref 2	ALKBH5 promotes colon cancer progression by decreasing methylation of the lncRNA NEAT1. Am J Transl Res. 2020 Aug 15;12(8):4542-4549. eCollection 2020.
Ref 3	METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation. Mol Cell. 2019 Jun 20;74(6):1278-1290.e9. doi: 10.1016/j.molcel.2019.03.040. Epub 2019 Apr 25.
Ref 4	Downregulation of lncRNA NEAT1 Ameliorates LPS-Induced Inflammatory Responses by Promoting Macrophage M2 Polarization via miR-125a-5p/TRAF6/TAK1 Axis. Inflammation. 2020 Aug;43(4):1548-1560. doi: 10.1007/s10753-020-01231-y.
Ref 5	Long noncoding RNA NEAT1 sponges miR?125a?5p to suppress cardiomyocyte apoptosis via BCL2L12. Mol Med Rep. 2019 May;19(5):4468-4474. doi: 10.3892/mmr.2019.10095. Epub 2019 Mar 27.