m6A-centered Crosstalk Information

Mechanism of Crosstalk between m6A Modification and Epigenetic Regulation

m6A Modification:
Crosstalk ID	M6ACROT00407				[1], [2], [3], [4], [5], [6], [7]
m⁶A modification GAS5 GAS5 YTHDF3 : m⁶A sites Indirect Inhibition RNA modification MIR21 MIR21 ADARB1 Methylation : modification sites
m6A Regulator	YTH domain-containing family protein 3 (YTHDF3)			READER	Regulator Info
m6A Target	Growth arrest specific 5 (GAS5)				Targert Gene
Epigenetic Regulation that have Cross-talk with This m6A Modification:
Epigenetic Regulation Type	RNA modification (RNAMod) >> Adenosine-to-Inosine editing (A-to-I)
Epigenetic Regulator	Double-stranded RNA-specific editase 1 (ADARB1)			WRITER	View Details
Regulated Target	MicroRNA 21 (MIR21)				View Details
Crosstalk Relationship	m6A → A-to-I			Inhibition
Crosstalk Mechanism	m6A modification indirectly impacts RNA modification through downstream signaling pathways
Crosstalk Summary	YTHDF3 interacts with Growth arrest specific 5 (GAS5), increasing its m6A level and inhibiting its physical interaction with MicroRNA 21 (MIR21), which was regulated by ADARB1-mediated A-to-I modification.
In-vitro Model	HCT 116	Colon carcinoma	Homo sapiens		CVCL_0291
	RKO	Colon carcinoma	Homo sapiens		CVCL_0504
	U-118MG	Astrocytoma	Homo sapiens		CVCL_0633

References

Ref 1	Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m(6)A reader YTHDF3. Mol Cancer. 2019 Oct 16;18(1):143. doi: 10.1186/s12943-019-1079-y.
Ref 2	Modulation of microRNA editing, expression and processing by ADAR2 deaminase in glioblastoma. Genome Biol. 2015 Jan 13;16(1):5. doi: 10.1186/s13059-014-0575-z.
Ref 3	The Role of ADAR1 and ADAR2 in the Regulation of miRNA-21 in Idiopathic Pulmonary Fibrosis. Lung. 2018 Aug;196(4):393-400. doi: 10.1007/s00408-018-0115-9. Epub 2018 Apr 10.
Ref 4	lncRNA GAS5 acts as a ceRNA for miR-21 in suppressing PDGF-bb-induced proliferation and migration in vascular smooth muscle cells. J Cell Biochem. 2019 Sep;120(9):15233-15240. doi: 10.1002/jcb.28789. Epub 2019 May 8.
Ref 5	Long Noncoding RNA GAS5 Acts As A Tumor Suppressor In Laryngeal Squamous Cell Carcinoma Via miR-21. Cancer Manag Res. 2019 Sep 17;11:8487-8498. doi: 10.2147/CMAR.S213690. eCollection 2019.
Ref 6	Long noncoding RNA GAS5 induces abdominal aortic aneurysm formation by promoting smooth muscle apoptosis. Theranostics. 2019 Jul 28;9(19):5558-5576. doi: 10.7150/thno.34463. eCollection 2019.
Ref 7	GAS5/miR-21 Axis as a Potential Target to Rescue ZCL-082-Induced Autophagy of Female Germline Stem Cells In?Vitro. Mol Ther Nucleic Acids. 2019 Sep 6;17:436-447. doi: 10.1016/j.omtn.2019.06.012. Epub 2019 Jun 28.